盐酸氨溴索对成年大鼠高氧肺损伤的影响

目的研究盐酸氨溴索对成年大鼠高氧肺损伤的影响。方法成年雄性SD大鼠48只,随机分为6组每组8只。对照组（0h组）吸入空气,其余5组氧舱内吸入100%浓度O2,根据吸氧时间不同分为24h组、48h组、72h组、96h组、盐酸氨溴索（ABX）组（吸氧96h）;ABX组予ABX 30mg/kg腹腔内注射1次/d。到达实验终点后颈动脉采血行动脉血气分析,测定血清及支气管肺泡灌洗液（BALF）中炎症因子水平,肺组织酶活性和Bax、Bcl-2蛋白表达。结果随高氧暴露时间延长,大鼠氧合指数呈逐渐下降趋势,96h达到最低值。血清及BALF中TNF-α、IL-1β水平逐渐增高,48h达到高峰,此后开始下降,IL-6在72h达高峰。肺组织Bax蛋白表达逐渐增多,Bcl-2表达逐渐下降。ABX干预组同96h组相比氧合指数增加（P〈0.01）,血清及BALF中TNF-α、IL-1β和IL-6水平下降（P〈0.01）,肺组织中Bax蛋白表达显著下降（P〈0.01）,Bcl-2蛋白表达显著增高（P〈0.01）。结论盐酸氨溴索通过抗炎、抗氧化及抗凋亡作用能够减轻成年大鼠高氧肺损伤。

Effect of ambroxol hydrochloride on hyperoxia-induced lung injury in adult rats

Objective To study the effect of ambroxol hydrochloride on hyperoxia-induced lung injury in adult rats.Methods Forty-eight male Sprague-Dawley（SD） adult rats were randomly divided into control group（0h group）,and 24,48,72,96h and ambroxol hydrochloride intraperitoneal（ABX） groups,8 in each group.Rats in control group received room air,those in 24,48,72 and 96h groups received 100% O2 in hyperbaric oxygen chamber,and ABX group received ABX injection（30mg/kg per day）.Carotid blood gas was analyzed and cytokine level in bronchoalveolar lavage fluid（BALF） was measured.Expression level of tumor necrosis factor-α（TNF-α）,IL-1β and IL-6 were measured respectively.Activity of enzyme and expression of Bax and Bcl-2 in lungs were detected.Results The oxygenation index decreased gradually with the increasing exposure time to oxygen and reached its minimum at 96h.The expression level of TNF-α and IL-1β in serum and BALF increased gradually and reached its peak at 48h,and then decreased gradually.The expression level of IL-6 reached its maximum at 72h.The expression of Bax was gradually increased while that of Bcl-2 in lungs was gradually decreased.The oxygenation index,expression levels of TNF-α,IL-1β,IL-6 and Bax in serum and BALF were significantly lower while the expression level of Bcl-2 in lungs was significantly higher in ABX group than in 96h group（P0.01）.Conclusion Ambroxol hydrochloride can attenuate hyperoxia-induced lung injury in adult rats through its anti-inflammatory,anti-oxidant and anti-apoptosis effects.