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| HBV核心抗原EGFP融合蛋白表达质粒的构建及在Hep G2细胞中的表达 | |
| 引用本文： | 徐宁,陈智,姚航平,羊正纲. HBV核心抗原EGFP融合蛋白表达质粒的构建及在Hep G2细胞中的表达[J]. 浙江预防医学, 2005, 17(1): 3-5 |
| 作者姓名： | 徐宁 陈智 姚航平 羊正纲 |
| 作者单位： | 浙江大学医学院附属第一医院传染病研究所、卫生部传染病重点实验室,浙江,杭州,310003 |
| 摘 要： | 目的探讨采用构建的质粒pHBc-EGFP转染HepG2细胞,瞬时表达HBV核心抗原和增强型绿荧光蛋白的可行性.方法克隆HBV核心抗原基因进入真核表达载体pEGFP-N1中,脂质体法转染HepG2细胞后在荧光显微镜下观察增强型绿荧光蛋白表达,并行RT-PCR及细胞免疫组化分析融合蛋白的表达.结果重组pHBc-EGFP质粒可在HepG2细胞内瞬时表达HBV核心抗原和增强型绿荧光蛋白融合蛋白.结论pHBc-EGFP质粒构建成功,增强型绿荧光蛋白的表达可报告HBV核心抗原表达. |
| 关 键 词： | HBV核心抗原 增强型绿荧光蛋白 瞬时表达 |
| 文章编号： | 1007-0931(2005)01-0003-03 |
| 修稿时间： | 2004-07-28 |
Construction of a Plasmid of HBV Core Antigen-EGFP Fusion Protein and Its Expression in Hep G2 Cell |
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| XU Ning,CHEN Zhi,YAO Hang-ping et al.. Construction of a Plasmid of HBV Core Antigen-EGFP Fusion Protein and Its Expression in Hep G2 Cell[J]. Zhejiang Journal of Preventive Medicine, 2005, 17(1): 3-5 | |
| Authors: | XU Ning CHEN Zhi YAO Hang-ping et al. |
| Abstract: | Objective To study the transient expression of HBV core antigen and enhanced green fluorescent protein (EGFP) in Hep G 2 cells transfected by a constructed plasmid (pHBc-EGFP). Methods The core antigen gene of HBV was cloned into eukaryotic expression vector pEGFP-N 1, with which Hep G 2 cells were transfected by liposome transfection method. The expression of fusion gene was analyzed by fluorescent microscopy, RT-PCR and immunohistochemistry method, respectively. Results Recombinant plasmid pHBc-EGFP expressed the HBV core antigen-EGFP fusion protein in Hep G 2 cells transiently. Conclusion pHBc-EGFP is constructed successfully, and the expression of EGFP can serve as an indicator reflecting the expression of HBV core antigen gene. |
| Keywords: | HBV core antigen Enhanced green fluorescent protein Transient expression |
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