成人骨髓间充质干细胞抑制异体淋巴细胞增殖的实验研究

目的研究成人骨髓间充质干细胞（BMMSCs）的免疫调节作用。方法从成人骨髓中分离和培养间充质干细胞，并通过形态的均一性及流式细胞术检测其表面标志以鉴定纯度。将分离培养的间充质干细胞接种于96孔板中，经丝裂霉素处理后，与异体淋巴细胞共同培养3d；并以单独培养的异体淋巴细胞作为对照组。各组经培养后加入植物血凝素（PHA）刺激72h，用MTT还原法测定细胞增殖率，观察成人骨髓BMMSCs对异体淋巴细胞增殖转化的影响。结果成人BMMSCs与PHA诱导的异体淋巴细胞共同培养组抑制了细胞增殖，其增殖转化抑制率为对照组的60．68％，配对t检验结果显示，两组差异有统计学意义（P〈0．01）。结论成人BMMSCs可以抑制PHA诱导的异体淋巴细胞增殖转化，对同种异体免疫反应具有负调节作用。

Adult human bone marrow-derived mesenchymal stem cells suppresses allogeneic lymphocytes proliferation

Objective To study the immunomodulatory functions of adult human bone marrow-derived mesenchymal stem cells in vitro.Methods Adult human BMMSCs were separated with Percoll((1.073) g/ml) and cultured in Dulbecco's Modified Eagle's Medium with low glucose containing 10% fetal calf serum.The purity of BMMSCs was identified with the spindle-fibroblastic morphology by microphotograph and the phenotypes were tested by fluorescence-activated cell sorter(FACS).BMMSCs were plated in 96-well plates.After treated with mitomycin,BMMSCs were co-cultured for 72 h with allogeneic lymphocytes isolated from peripheral blood.Effects of MSCs on PHA induced lymphocytes transformation were investigated.The proliferation of lymphocytes was measured by MTT method.Results Peripheral blood lymphocytes proliferation was suppressed by BMMSCs and the inhibition ratio was (60.68%)(P<(0.01)).Conclusion BMMSCs pretreatment might be useful in the prevention of GVHD in HLA-mismatched hematopoietic stem cells transplantation and further,donors for hematopoietic stem cells could be selected from greater potentials.