西洋参茎叶总皂苷对心肌梗死大鼠非梗死区组织的保护作用

目的观察西洋参茎叶总皂苷(PQS)对急性心肌梗死(AMI)大鼠非梗死区组织的保护作用。方法选择Wistar大鼠100只建立AMI模型,造模后随机分为模型组20只、美托洛尔组20只、PQS组20只、正常组20只和假手术组20只。光镜观察大鼠缺血及非缺血区心肌组织病理变化;ELISA法检测高敏C反应蛋白含量;放射免疫法检测白细胞介素6、内皮素1水平;硝酸还原酶法检测NO变化;比色法检测细胞色素C氧化酶及ATP合成酶活性。光镜观察正常组与模型组大鼠肾脏组织病理变化。结果光镜下可见缺血区和非缺血区心肌不同程度炎性细胞浸润、心肌纤维变形等组织损伤改变。与模型组比较,PQS组及美托洛尔组缺血区及非缺血区心肌高敏C反应蛋白、白细胞介素6、内皮素1明显降低,NO明显升高(P<0.05,P<0.01)。光镜观察显示,模型组大鼠肾脏组织毛细血管壁明显增厚,肾间质可见较多的中性粒细胞及单核细胞浸润。结论 PQS能够通过抗炎、保护血管内皮、调节能量代谢等途径保护AMI后受损的非缺血区心肌组织。

Protective effect of Panax quinquefolius saponin on non-ischemic myocardium in myocardial infarction rats

Objective To observe the protective effect of PQS on non-ischemic myocardium in AMI rats.Methods One hudred Wistar rats were included in this study.After a rat AMI modelwas established by ligation of the left anterior ascending coronary artery,the rats were randomly divided into model group,metoprolol group,PQS group,normal control group and sham operation group (20 in each group).Pathological changes in ischemic and non-ischemic myocardial tissues of rats were observed under light microscope.hs-CRP was detected by ELISA.IL-6 and ET-1 levels were measured by radioimmunoassay.Changes in NO were detected with biochemical method.Biological activity of cytochrome C oxidase and ATP synthase was assayed by colorimetry.Pathological changes in renal tissues from normal control and model groups were observed under optical microscope. Results Different extents of inflammatory cell infiltration and myocardial damages such as myocardial fiber distortion were observed in ischemic and non-ischemic myocardium.The hsvCRP, IL-6 and ET-1 levels were significantly lower while the NO level was significantly higher in PQS group and metoprolol group than in model group(F<0.05,P<0.01).Light microscopy showed that the renal tissue capillary wall was significantly thicker in model group with more neutrophils and monocytes observed in renal interstrtium.Conclusion PQS can protect non-ischemic myocardium against damage following AMI by antagozing inflammatory reaction,protecting blood vessel endothelium and regulating energy metabolim.