| The Gene Expression Patterns of Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus |
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| 引用本文： | 李守新,江薇,黄睿,王晓慧,刘文,沈寿引. The Gene Expression Patterns of Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus[J]. 华中科技大学学报(医学英德文版), 2007, 27(4): 367-371. DOI: 10.1007/s11596-007-0405-6 |
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| 作者姓名： | 李守新 江薇 黄睿 王晓慧 刘文 沈寿引 |
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| 作者单位： | Department of Rheumatology Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Rheumatology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Rheumatology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Rheumatology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Rheumatology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Rheumatology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China |
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | This study examined the gene expression patterns of peripheral blood mononuclear cells(PBMCs) in patients with systemic lupus erythematosus(SLE) by using serial analysis of gene ex-pression(SAGE) technology.Following the construction of serial analysis of gene expression(SAGE) library of PBMCs collected from 3 cases of familial SLE patients,a large scale of tag sequencing was performed.The data extracted from sequencing files was analyzed with SAGE 2000 V 4.5 software.The top 30 expressed genes of SLE patients were uploaded to http://david.niaid.nih.gov/david/ease.htm and the functional classification of genes was obtained.The differences among those expressed gene were analyzed by Chi-square tests.The results showed that a total of 1286 unique SAGE tags were identified from 1814 individual SAGE tags.Among the 1286 unique tags,86.8% had single copy,and only 0.2% tags had more than 20 copies.And 68.4% of the tags matched known expressed sequences,41.1% of which matched more than one known expressed sequence.About 31.6% of the tags had no match and could represent potentially novel genes.Ap-proximately one third of the top 30 genes were ribosomal protein,and the rest were genes related to metabolism or with unknown functions.Eight tags were found to express differentially in SAGE li-brary of SLE patients.This study draws a profile of gene expression patterns of PBMCs in patients with SLE.Comparison of SAGE database from PBMCs between normal individuals and SLE pa-tients will help us to better understand the pathogenesis of SLE.
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| 关 键 词： | 系统性红斑狼疮 基因表达 外周血 单核细胞 |
| 收稿时间： | 2003-11-04 |
| 修稿时间： | 2007-01-09 |
The gene expression patterns of peripheral blood mononuclear cells in patients with systemic lupus erythematosus |
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| Li Shouxin,Jiang Wei,Huang Rui,Wang Xiaohui,Liu Wen,Shen Shouyin. The gene expression patterns of peripheral blood mononuclear cells in patients with systemic lupus erythematosus[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2007, 27(4): 367-371. DOI: 10.1007/s11596-007-0405-6 |
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| Authors: | Li Shouxin Jiang Wei Huang Rui Wang Xiaohui Liu Wen Shen Shouyin |
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| Affiliation: | Department of Rheumatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | This study examined the gene expression patterns of peripheral blood mononuclear cells(PBMCs) in patients with systemic lupus erythematosus(SLE) by using serial analysis of gene ex-pression(SAGE) technology.Following the construction of serial analysis of gene expression(SAGE) library of PBMCs collected from 3 cases of familial SLE patients,a large scale of tag sequencing was performed.The data extracted from sequencing files was analyzed with SAGE 2000 V 4.5 software.The top 30 expressed genes of SLE patients were uploaded to http://david.niaid.nih.gov/david/ease.htm and the functional classification of genes was obtained.The differences among those expressed gene were analyzed by Chi-square tests.The results showed that a total of 1286 unique SAGE tags were identified from 1814 individual SAGE tags.Among the 1286 unique tags,86.8% had single copy,and only 0.2% tags had more than 20 copies.And 68.4% of the tags matched known expressed sequences,41.1% of which matched more than one known expressed sequence.About 31.6% of the tags had no match and could represent potentially novel genes.Ap-proximately one third of the top 30 genes were ribosomal protein,and the rest were genes related to metabolism or with unknown functions.Eight tags were found to express differentially in SAGE li-brary of SLE patients.This study draws a profile of gene expression patterns of PBMCs in patients with SLE.Comparison of SAGE database from PBMCs between normal individuals and SLE pa-tients will help us to better understand the pathogenesis of SLE. |
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| Keywords: | systemic lupus erythematosus of serial analysis gene expression peripheral blood mononuclear cells |
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