JAK激酶抑制剂AG490对乳腺癌细胞侵袭转移的影响

目的研究JAK激酶抑制剂AG490对乳腺癌细胞MDA-MB-231侵袭转移的影响,探讨JAK-STAT3信号转导通路在乳腺癌侵袭转移调控中的作用。方法以乳腺癌细胞MDA-MB-231为研究对象,应用JAK激酶抑制剂AG490处理细胞,用MTT法检测细胞对人工基底膜的粘附能力变化;Transwell小室进行人工重组基底膜侵袭和运动实验;Western blot检测细胞中P-STAT3水平。结果JAK激酶抑制剂AG490可使人乳腺癌细胞MDA-MB-231中P-STAT3表达减弱,同时可使细胞粘附、侵袭、迁移能力下降。结论JAK-STAT3信号转导通路参与乳腺癌细胞侵袭转移调控,阻断通路活化可以抑制乳腺癌细胞的侵袭转移。

Effect of Janus Kinase Inhibitor AG490 on Invasion and Metastasis of Human Breast Cancer Cells

Objective To investigate the effect of Janus kinase(JAK)inhibitor AG490 on invasion and metastasis of the human breast cancer cell MDA-MB-231,and to explore the regulating role of JAK-STAT3 signaling pathway when the breast cancer occurs to the invasiveness and metastasis.Methods The human breast cancer cell MDA-MB-231 was used as the research object,and AG490 was as Janus kinase inhibitor.The adhesion of MDA-MB-231 cell attaching to matrigel was measured with MTT assay.The invasion and metastasis potential were evaluated with transwell chamber.The P-STAT3 protein in cell was detected by Western-blot.Results Janus kinase inhibitor AG490 could make that the expression of P-STAT3 in human breast cancer cell MDA-MB-231 became weak,and that the abilities of adhesion,invasion and metastasis were also dropping down as compared with the control group(P<0.01).Conclusion JAK-STAT3 signaling pathway participates in regulating the invasion and metastasis of human breast cancer.Inhibiting the activation of JAK-STAT3 signaling pathway can suppress the invasion and metastasis of human breast cancer.