Evaluation of the Mycoplasma Duo kit for the detection of Mycoplasma hominis and Ureaplasma urealyticum from urogenital and placental specimens

This study compares the Mycoplasma Duo kit for the detection of genital mycoplasmas with conventional culture using A7 differential agar for the detection of Mycoplasma hominis and Ureaplasma urealyticum in clinical samples. Detection of the mycoplasmas is based on the specific metabolic properties of each organism to hydrolyse either arginine or urea. The Mycoplasma Duo test showed a significantly higher detection rate than did culture, although many of the culture-negative results may have been due to the presence of bacterial overgrowth.