层粘连蛋白对腺样囊性癌细胞侵袭和转移的影响

目的 研究层粘连蛋白（ＬＮ）与人涎腺腺样囊性癌转移的关系。方法 应用免疫组织化学染色，Ｂｏｙｄｅｎ小室体外移动试验和体外粘附试验，对人涎腺腺样囊性癌细胞系Ａｃｃ－２和其克隆出的肺高转移细胞株Ａｃｃ－Ｍ进行比较研究。结果 发现ＬＮ和层粘连蛋白受体（ＬＮＲ）在Ａｃｃ－Ｍ表达明显高于Ａｃｃ－２。在加入外源性ＬＮ后，Ａｃｃ－２体外移动性、粘附率均明显提高，而Ａｃｃ－Ｍ无明显变化。结论 层粘连蛋白在Ａｃｃ转

Effects of laminin on the invasive and metastatic behaviors of human salivary adenoid cystic carcinoma cell lines

Objective To study the effect of laminin(LN) on invasive and metastastic behaviors of salivary adenoid cystic carcinoma(Acc) cell lines.Methods Immunohistochemistry, Boyden chamber in vitro motility, and adhesion test were used. LN and LNR(LN receptor) distributions in Acc 2 and Acc M cells were studied with immunohistochemical staining.Results There were less LN and LNR found in Acc 2, but much more LN and LNR were present in the highy metastatic clone Acc M. With the presence of exgenous LN, Acc 2 became activated showing a higher migration rate. The cell adhesion test showed that Acc 2 had stronger ability of adhesion than Acc M with the presence of LN.Conclusion The result suggests LN and LNR may play a vitral role in the invasion and metastasis of Acc.