The expression of miR-375 in prostate cancer: A study based on GEO,TCGA data and bioinformatics analysis |
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| Affiliation: | 1. Department of Epidemiology and Health Statistics, Xiangya School of Public Health, Central South University, Shangmayuanling 238, Kaifu District, Changsha, Hunan 410078, People’s Republic of China;2. Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, People’s Republic of China;1. Laboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and Technology, Chenggong Campus, 727 South Jingming Road, Kunming City, Yunnan Province 650500, China;2. Yunnan Provincial Institute of Digestive Medicine, The First People''s Hospital of Yunnan Province, No. 157, Jinbi Road, Xishan District, Kunming City, Yunnan Province 650500, China;1. Department of Pathology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region 530021, PR China;2. Department of Medical Oncology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region 530021, PR China;3. Department of Radiology, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region 530021, PR China;1. Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China;2. Department of Radiotherapy, Shantou Central Hospital, Shantou, 515031, China;1. Center for Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University, People’s Republic of China;2. Key Laboratory for Reproductive Medicine of Guangdong, People’s Republic of China;3. Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, People’s Republic of China;4. Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, People’s Republic of China;1. Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 214062, China;2. Department of Oncological Surgery, The Affiliated Hospital of Jiangnan University, Wuxi, 214062, China;1. Department of Medical Oncology, The First Hospital of China Medical University, Shenyang 110001, China;2. Key Laboratory of Anticancer Drugs and Biotherapy of Liaoning Province, The First Hospital of China Medical University, Shenyang 110001, China |
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| Abstract: | BackgroundMiR-375, as a member of miRNA family, plays essential roles in prostate cancer (PC). We purpose to explore the expression and possible molecular mechanism of the miR-375 in PC using database analysis.MethodsFirst, Student’s t-test, overall and subgroup meta-analyses with 20 eligible datasets in the Gene Expression Omnibus (GEO) database were performed to explore the expression of miR-375 in PC. Then the results of meta-analyses were verified in The Cancer Genome Atlas (TCGA) database by Student’s t-test and Paired t-test. The candidate genes of miR-375 were predicted by four platforms. Protein-protein interaction (PPI) networks, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to investigate the potential molecular mechanism of miR-375 in PC.ResultsThe overall meta-analysis showed the expression of miR-375 was significantly up-regulated in PC groups compared with non-cancerous group (SMD; 0.71; 95% CI: 0.38–1.04). In addition, the meta-analyses by subgroup showed the expression of miR-375 in PC tissues was higher than that in healthy prostate tissues and adjacent non-cancerous tissues. The results of TCGA database verified the expression of miR-375 in PC tissues was obviously higher than that in adjacent non-cancerous tissues. Moreover, GO and KEGG analysis revealed that the latent target genes were mainly involved in protein binding function and ubiquitin mediated proteolysis. PPI analysis identified JAK2, EHMT1 and QKI as the hub genes (highly connected genes with high degree in PPI).ConclusionsMiR-375 was up-regulated in PC tissues. Meanwhile, miR-375 may play an important role in aggressive PC by targeting its potential target genes. |
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| Keywords: | Prostate cancer miR-375 Meta-analysis Gene expression omnibus The cancer genome atlas |
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