长链非编码RNA TSI抑制TGF-β1介导的乳腺癌细胞MCF-7和BT474转移

目的 研究长链非编码RNA TSI（Lnc-TSI）在TGF-β1促进乳腺癌细胞MCF-7和BT474转移中的作用。方法 TGF-β1处理乳腺癌细胞MCF-7和BT474后,观察细胞形态变化,western blot实验检测上皮-间充质转化相关蛋白的变化,transwell实验检测细胞侵袭能力。qRT-PCR分别检测MCF-10A和TGF-β1处理前后MCF-7、BT474细胞的Lnc-TSI变化。在MCF-7和BT474细胞中分别敲除和过表达Lnc-TSI,transwell实验检测细胞在TGF-β1处理后侵袭迁移能力的变化。结果 TGF-β1引起乳腺癌细胞MCF-7和BT474发生上皮-间充质转化,细胞形态呈梭形改变,上皮-间充质转化相关蛋白E-cadherin下调,N-cadherin和Vimentin上调,细胞侵袭数目增加。与正常乳腺上皮细胞MCF-10A细胞相比,MCF-7和BT474细胞中的Lnc-TSI表达更高。TGF-β1处理能显著上调MCF-7和BT474细胞中的Lnc-TSI水平。Transwell实验显示敲除和过表达Lnc-TSI能分别增强和抑制MCF-7和BT474细胞的侵袭和迁移,在TGF-β1处理下这种现象更为明显。结论 在TGF-β1促进的乳腺癌细胞MCF-7和BT474的转移进程中Lnc-TSI表达上调,Lnc-TSI能抑制乳腺癌细胞MCF-7和BT474的转移。

Long non-coding RNA TSI inhibited the metastasis induced by TGF-β1 in breast cencer cell MCF-7 and BT474

Objective To uncover the function of long non-coding RNA TSI （Lnc-TSI） in the TGF-β₁ induced metastasis of breast cancer cell MCF-7 and BT474. Methods We treated the MCF-7 and BT474 cells with TGF-β1 and investigated the morphological changes. Epithelial-mesenchymal transition （EMT） associated proteins were measured with western blot and cell invasion were tested with transwell assay. And then qPCR was used toverify the expression of Lnc-TSI in MCF-10A cells, compared with MCF-7 and BT474 cells with or without TGF-β1 treatment. After knocking out and overexpressing Lnc-TSI in MCF-7 and BT474 cells respectively, we conducted transwell assay to see the changes of cellmigration and invasion. Results Morphology scale showed highly possible EMT changes in MCF-7 and BT474 cells. Also, EMT associated protein E-cadherin were down-regulated while N-cadherin and Vimentin were up-regulated, consisting with the enhanced invasion after treating with TGF-β1. Compared with MCF-10A cells, Lnc-TSI level was much higher in MCF-7 and BT474 cells. TGF-β1 dramatically up-regulated the expression of Lnc-TSI in MCF-7 and BT474 cells. Knocking out and overexpressing Lnc-TSI in MCF-7 and BT474 cells were able to enhanced and inhibited the cell migration and invasion respectively, which were both more obvious with TGF-β1 treatment. Conclusion Lnc-TSI was up-regulated following the metastasis mediated by TGF-β1 in MCF-7 and BT474 cells. Lnc-TSI suppressed the metastasis of MCF-7 and BT474 cells.