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| 小脑变性相关蛋白2 mRNA在癫痫患者脑组织中的表达及其临床意义 | |
| 引用本文： | 席志芹,王学峰,龚云,李劲梅,汪建华,孙纪军,龚玉来,晏勇,郭珍立,栾国明,王玉平,李云林,张建国,卢涌,李红卫.小脑变性相关蛋白2 mRNA在癫痫患者脑组织中的表达及其临床意义[J].中国神经免疫学和神经病学杂志,2006,13(6):364-368. |
| 作者姓名： | 席志芹 王学峰 龚云 李劲梅 汪建华 孙纪军 龚玉来 晏勇 郭珍立 栾国明 王玉平 李云林 张建国 卢涌 李红卫 |
| 作者单位： | 1. 重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室,重庆400016 2. 武警四川省总队医院神经内科,四川 乐山 614000 3. 武汉新华脑科中心 湖北 武汉430015 4. 首都医科大学天坛医院,北京100050 5. 首都医科大学宣武医院神经外科,北京100053 6. 重庆刑事科学技术研究所,重庆400016 |
| 摘 要： | 目的了解小脑变性相关蛋白2(CDR2)mRNA在癫痫(EP)患者脑组织中的表达及其临床意义。方法将48例EP患者分成耐药(40例)和非耐药(8例)两组,用基因芯片扫描和FQ-PCR分别检测脑组织中CDR2基因表达,并与对照组比较。结果基因芯片扫描提示CDR2 mRNA在EP患者与对照组中的表达差异有统计学意义(P<0.05)。FQ-PCR结果与基因芯片扫描一致。结论EP患者脑组织中有CDR2 mRNA表达,由于其表达产物可通过血-脑脊液屏障进入脑脊液(CSF)和血液中,因而检测CDR2 mRNA在血或CSF中的表达产物有可能成为诊断EP的一个重要指标。 |
| 关 键 词： | 癫痫 小脑变性相关蛋白2 基因芯片 |
| 文章编号： | 1006-2963（2006）06-0364-05 |
| 收稿时间： | 2005-09-26 |
| 修稿时间： | 2006-01-10 |
Expression of Cerebellar Degeneration-related Protein 2 mRNA in Brain Tissue of Patients with Epilepsy and Its Clinical Significance |
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| XI Zhi-qin,WANG Xue-feng,GONG Yun,LI Jin-mei,WANG Jian-hua,SUN Ji-jun,GONG Yu-lai,YAN Yong,GUO Zhen-Li,LUAN Guo-ming,WANG Yu-ping,LI Yun-lin,ZHANG Jian-guo,LU Yong,LI Hong-wei.Expression of Cerebellar Degeneration-related Protein 2 mRNA in Brain Tissue of Patients with Epilepsy and Its Clinical Significance[J].Chinese Journal of Neuroimmunology and Neurology,2006,13(6):364-368. | |
| Authors: | XI Zhi-qin WANG Xue-feng GONG Yun LI Jin-mei WANG Jian-hua SUN Ji-jun GONG Yu-lai YAN Yong GUO Zhen-Li LUAN Guo-ming WANG Yu-ping LI Yun-lin ZHANG Jian-guo LU Yong LI Hong-wei |
| Institution: | 1. Department of Neurology, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016 ;2. Department of Neurology, Sichuan Provincial Corps Hospital, Sichuan Leshan 614000; 3. Department of Neurology, Xinhua Hospital, Hubei Wuhan 430015 ; 4. Department of Neurology, Beijing Tiantan Hospital, Beijing 100050 ;5. Department of Neurosurgery, Xuanwu Hospital, Capital University of Medical Sciences,Beijing 100053 ; 6. Chongqing Criminal Science and Technology Institute, Chongqing 400016, China |
| Abstract: | Objective To investigate the expression of cerebellar degeneration-related protein 2(CDR2) mRNA in brain tissue of patients with epilepsy and its clinical significance. Methods The CDR2 mRNA was detected by Gene microarray screening and real-time fluorescence quantitative PCR in brain tissue of 40 patients with intractable epilepsy(IE) and 8 with non-intractable epilepsy(NIE).Results The CDR2 mRNA level was significant higher in brain tissue of patients with epilepsy,especially in non-intractable epileptic patients than that in the control group.The results of FQ-PCR were coincided well with those of cDNA microarray.Conclusions The gene expression patterns of CDR2 in samples of epileptic patients are distinct from those of controls'.The product of CDR2 can pass through blood brain barrier into blood and cerebrospinal fluid,and be detected in peripheral blood and cerebrospinal fluid,and be used as a important diagnostic parameter for epilepsy. |
| Keywords: | FQ-PCR |
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