甲醛对人外周血淋巴细胞遗传毒性的研究

目的:研究甲醛对人外周血淋巴细胞染色体的影响.方法:用不同浓度(5、25、125、500μmol/L)的甲醛溶液处理培养中的人外周血淋巴细胞,观察其微核率及SCE频率的变化.结果:低浓度(5、25 μmol/L)甲醛处理后,人外周血淋巴细胞微核率和SCE频率与阴性对照组相比,无明显增加(P＞0.05);而甲醛浓度(125、500 μmol/L)较高时,细胞微核率和SCE频率均显著高于阴性对照组(P＜0.05或P ＜0.01).结论:较高浓度甲醛对人外周血淋巴细胞染色体有不同程度的损伤作用.

Studies on Genotoxicity of Formaldehyde on Human Peripheral Lymphocyte

Objective:Effects of formaldehyde on peripheral lymphocyte chromosome out of human body was studied.Methods:Lymphocytes in culture fluid were treated by supplementing different doses(5、25、125、500 μmol/L) of formaldehyde,then the changes of micronucleus and SCE rates were observed.Results:It is found that no significant increment in both Micronucleus and SCE rates were detected at low doses of formaldehyde(5、25 μmol/L)in peripheral lymphocytes(P>0.05);Micronucleus and SCE rates of test group were obviously higher than those of the negative control(P<0.05 or P<0.01)at the higher doses(125、500 μmol/L).Conclusion:formaldehyde may exert different degree of damage to peripheral lymphocyte chromosome out of human body.