荧光多重PCR与血清型特异性抗体检测HSV感染的比较

目的：比较荧光多重PCR和血清型特异性抗体测定在生殖器疱疹临床诊断中的应用价值及评价各自的优缺点。方法：以细胞培养法作为“金标准”对照，分别用荧光多重PCR和血清型特异性抗体检测法对121例临床诊断为生殖器疱疹的标本进行检测。结果：以培养法作标准，并通过结果的差异性分析，荧光多重PCR的敏感性为100％，特异性为88．89％；血清型特异性抗体测定则分别为77．68％和77．78％，荧光多重PCR的敏感性和特异性均显高于血清型特异性抗体测（P＜0．05），但前不能检测出无皮损患HSV的DNA，而后可检测出无皮损患中的HSV抗体。结论：荧光多重PCR和血清型特异性抗体检测各有其自身的优缺点，单独用PCR和其它病毒分离的方法和单独使用血清特异性抗体检测的方法来诊断生殖器疱疹都是不完整的，均可造成漏诊。临床上将两种方法有机的结合起来应用能发挥各自的优势，取长补短，对早期、准确、快速地诊断生殖器疱疹及进行流行病学调查有着十分重要的意义和使用价值。

Comparison of Multiplex Fluorescent PCR with Serum Type - specific Antibody Detection in Diagnosis of Genital Herpes

Objective:To compare multiplex fluorescent PCR with serum type-specific antibody detection in diagnosis of genital herpes and evaluate it's significance in diagnosis of genital herpes. Methods:We detected 121 specimens collected from the patients with genital herpes using either the multiplex fluorescent PCR and serum type-specific antibody detection. HSV viral isolation was as the standard.Results:Compared with viral isolation, the sensitivity, specificity for multiplex fluorescent PCR was 100% and 88.89%, for type-specific antibody detection was 77.68% and 77.78% respectively. Contrast to 2 positive results of type-specific antibody detection in two asymptomatic patients, the multiplex fluorescent PCR couldn't detect any HSV DNA from those asymptomatic patients. Conclusion:While multiplex fluorescent PCR was a sensitive and specific method for detection and typing of HSV in the lesion of genital herpes, but failed to detect HSV DNA from the asymptomatic patients, serum type-specific antibody detection could detected the specific antibody from some of those asymptomatic patients. Combination of these two techniques would allow rapid, sensitive and accurate detection and typing of HSV and help clinical diagnosis and epidemiologic survey of genital herpes.