| HPLC测定小儿退热口服液中绿原酸与黄芩苷含量 |
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| 引用本文： | 范全民. HPLC测定小儿退热口服液中绿原酸与黄芩苷含量[J]. 现代中药研究与实践, 2011, 0(2): 72-74 |
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| 作者姓名： | 范全民 |
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| 作者单位： | 河南省济源市食品药品检验所; |
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| 摘 要： | 目的 建立高效液相色谱法同时测定小儿退热口服液中绿原酸和黄芩苷的含量方法.方法 采用VP-ODS C<,18>色谱柱(150 mm×4.6 mm,5 μm),流动相:甲醇-0.2%磷酸溶液,流速:1.0 mL·min<'-1>,检测波长为327 nm,柱温:20℃.结果 绿原酸在23.2～92.8 μg·mL<'-1>...
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| 关 键 词： | HPLC 小儿退热口服液 绿原酸 黄芩苷 |
Determination of Chlorogenic Acid and Baicalin in Xiaoer Tuire Koufuye by HPLC |
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| FAN Quan-min. Determination of Chlorogenic Acid and Baicalin in Xiaoer Tuire Koufuye by HPLC[J]. Research and Practice on Chinese Medicines, 2011, 0(2): 72-74 |
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| Authors: | FAN Quan-min |
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| Affiliation: | FAN Quan-min(Jiyuan Institute for Food and Drug Control,Jiyuan 454650,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of chlorogenic acid and baicalin in Xiaoer Tuire Koufuye.Methods The method was performed on VP-ODS C18 column（150 mm×4.6 mm,5 μm）,with mobile phase consisting of methanol and 0.2% phosphoric acid.The flow rate was 1.0 mL·min-1,detection wavelength was set at 327 nm.Results The calibration curve showed a good linearity with the range of 23.2~92.8 μg·mL-1 for chlorogenic acid,the average recovery was 98.0%,with RSD of 1.0%,and had a good linearity with the range of 69.2~276.8 μg·mL-1 for baicalin,the average recovery was 98.2% with RSD of 1.2%.Conclusion The method is simple,accurate and repeatable,it can be used for the quality control of the preparation. |
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| Keywords: | Xiaoer Tuire Koufuye HPLC chlorogenic acid baicalin |
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