风动式甲醛熏蒸导管灭菌效果研究杨淑萍

目的提高甲醛熏蒸灭菌效果，缩短消毒时间。方法采用风动式甲醛熏蒸灭菌法，将内外壁遍涂菌液后待干的3种内径导管作为消毒效果观察点，取0.5h、1h、1.5h、2h、3h样本，按无菌操作法剪下导管中段4cm，种入无菌肉汤试管内，放入35℃恒温箱内培养48h，做细菌学鉴定，重复5次；普通熏蒸法采用同样时间点，相同导管样本做为对照组；结果风动式甲醛熏蒸灭菌法3种口径导管在消毒0.5h时，细菌阳性率低于50%，1h时即达灭菌效果。普通熏蒸法0.5h100%有菌生长，1h细菌阳性率80%以上，3h方达同样灭菌效果。两种灭菌方法效果比较，差异有高度显著性(P<0.001)。结论风动式甲醛熏蒸灭菌较普通法所需时间短，灭菌效果完全。

Effects of wind formaldehyde fumigation

Objective:To improve the effect of formaldehyde fumigation sterilization and t o shorten the time of the sterlization.Methods:The evperiaeutal group un der went wind formadelyde fumigation.The interior and the exterior of catheters of three different calibese were smeared with bacterial fluid and the steril iza tion effects were observed .Sampling was performed at 0.5h,1h,1.5h,2h and 3h .4cm sampler were taken from the catheter using aseptic manipulation an d placed into ase p tic broth.Samples were placed in a thermostated bath at 35℃ for 48h.The test was repeated 5 times and bacteria were identified.Samples taken at the same time f rom the same catheters that received ordinary fumigation sterihzation served as controls.Results:The catheters receiving wind formaldehyde fumigation showed.5 0% less bacteria growth at 0.5h and had bacterial growth at 1h.By ordina ry fumigation,there was 100% growth of bacteria at 0.5h and more than 80% at 1h.There was no growth at 3h.The difference between the two methods was sterilization ( P<0.01).Conclusion:Sterilization by wind formaldehyde fumigation can shorten the time ard improve the effect of sterilization compared with ordinary fumigation.