自身免疫性血小板减少性紫癜患者外周血中CD4+CD25+调节性T细胞、sFas和sFasL的表达及临床意义

本研究通过检测成人自身免疫性血小板减少性紫癜(AITP)外周血中CI4+CD25+调节性T细胞(Treg)、sFas和sFasL的表达,探讨它们在AITP发病机制中的作用及临床意义,为寻求AITP治疗的有效方法提供理论依据.采用流式细胞术分别检测30例AITP患者和18例正常对照者外周血CD4+T、Treg、CD4+ CD25-T细胞表达率及Treg/CD4+T比值；用酶联免疫吸附法(ELISA)检测AITP患者治疗前后及对照组外周血sFas、sFasL表达水平.结果表明,AITP组外周血中CD4+T细胞表达率低于对照组(p＜0.05),Treg细胞表达率及Treg/CD4+T比值明显低于正常对照组(p＜0.0l).AITP组患者治疗前外周血中sFas和sFasL水平明显高于治疗后和正常对照组(p＜0.01),AITP组治疗后与正常对照组外周血sFas、sFasL水平差异无统计学意义(p＞0.05).AITP患者治疗前Treg细胞表达率、Treg/CD4+T细胞比值与血小板计数呈正相关；AITP患者外周血中sFas和sFasL水平呈正相关；CD4+T细胞、CD4+CD25 -T细胞表达率,sFas、sFasL浓度与血小板计数无明显相关；Treg细胞表达率和sFas、sFasL浓度间没有明显相关性.结论:Treg在AITP的发病机制中发挥一定作用；Treg细胞水平与AITP病情的严重程度有关；sFas和sFasL水平异常参与了AITP的免疫病理过程.

Expression and Clinical Significance of CD4 + CD25 + Treg Cells, sFas and sFasL in Peripheral Blood of Patients with Autoimmune Thrombocytopenic Purpura

This study was aimed to detect the expression of CD4+CD25+ regulatory T cells(Treg),sFas and sFasL in patients with autoimmune thrombocytopenic purpura(AITP),and to explore their roles in the pathogenesis of AITP and clinical significance,so as to provie a theoretical basis for effective treatment for AITP.The expressions of CD4+T,Treg,CD4+CD25-T,Treg /CD4+T in peripheral blood of 30 the patients with AITP and 18 controls were detected by flow cytometry,and enzyme-linked immunosorbent assay(ELISA) was used ...