实时定量逆转录PCR检测慢性淋巴细胞白血病MDM4基因mRNA的表达及其临床意义

本研究旨在探讨慢性淋巴细胞白血病(CLL)患者鼠双微体4(MDM4)基因mRNA的表达情况及其在CLL预后中的意义.采用SYBRGreenⅠ荧光染料行实时定量逆转录PCR(qRT-PCR)方法检测66例CLL患者白血病细胞MDM4 mRNA的表达,以β-actin为内参照物,以2(-△Ct)方法计算MDM4的相对定量值...

mRNA levels detected by real-time quantitative RT-PCR in chronic lymphocytic leukemia and their clinical significance

This study was aimed to investigate the expression level of murine double minute 4（MDM4） mRNA in chronic lymphocytic leukemia（CLL） and its prognostic value in CLL.By means of β-actin as internal reference,the real-time quantitative RT-PCR was set up.The expression of MDM4 mRNA in 66 CLL patients was measured by fluorescence dye SYBR Green I.The dispersion of MDM4 expression ratio of groups with different prognostic factors was described by using Mann-Whitney U test.The results showed that the median MDM4 mRNA expression level was 0.037098（0.088245-0.014875） in CLL patients.The expression level of MDM4 mRNA was significantly higher in patients with P53 gene deletion than that in patients without P53 gene deletion（0.13167 vs 0.030927）（p0.001）,and also significantly higher in patients with P53 mutation than that in patients without P53 mutation（0.13167 vs 0.03077）（p0.001）.MDM4 expression was also associated with Binet stages（p=0.044） and ATM gene deletion（p=0.046）,but was not associated with LDH（p=0.216）,β2-MG（p=0.314）,TK1（p=0.300）,ZAP-70（p=0.559）,CD38（p=0.513） and IgVH mutation status（p=0.333）.It is concluded that the expression level of MDM4 is significantly higher in patients with P53 deletion or mutation.MDM4 expression is significantly associated with Binet stages and ATM gene deletion.MDM4 may be an important prognostic factor in CLL.