芦荟凝胶对多柔比星外渗性损伤的防治及其机制初探

目的观察芦荟凝胶对大鼠多柔比星外渗性损伤的防治作用,初步探讨芦荟凝胶促进大鼠多柔比星外渗性损伤伤口愈合的机制。方法建立SD大鼠多柔比星外渗性损伤动物模型,并随机分为正常组、多柔比星组和芦荟凝胶组（芦荟凝胶0.1、1.0 g或10.0 g/L）。测量外渗性损伤的面积以观察损伤程度,HE染色观察病理形态学改变,Western bloting检测损伤部位的皮肤及皮下组织中bcl-2和caspase-3的表达情况。结果多柔比星外渗性损伤11天后其损伤面积达最大值;芦荟凝胶能浓度依赖性地减轻多柔比星作用11天后的外渗性损伤面积（P〈0.01）。HE染色结果显示：不同浓度的芦荟凝胶组大鼠外渗性损伤处其损伤程度轻于多柔比星组。多柔比星能显著抑制皮肤及皮下组织中的bcl-2蛋白表达,芦荟凝胶能显著促进皮肤及皮下组织中的bcl-2蛋白表达,并减轻多柔比星对皮肤及皮下组织中bcl-2蛋白表达的抑制作用。多柔比星能显著促进皮肤及皮下组织中的caspase-3蛋白的表达,芦荟凝胶本身对caspase-3蛋白的表达无明显作用,但可显著抑制多柔比星对皮肤及皮下组织中caspase-3蛋白表达的上调作用。结论芦荟凝胶对大鼠多柔比星外渗性损伤有良好的防治作用,其机制可能与其促进bcl-2的表达和抑制caspase-3的表达有关。

Aloe Gel Inhibits Doxorubicin-Induced Extravasation Injury in Rats by Up-Regulating Expression of Bcl-2 and Down-Regulating Expression of Caspase-3

Objective To investigate the preventive effect of aloe gel on doxorubicin-induced extravasation injury and the underlying mechanism. Methods Sprague-Dawley(SD) mice were used to establish the extravasation injury model induced by doxorubicin.Fifty SD mice were randomly divided into five groups: non-injured control group,doxorubicin-induced extravasation injured group,aloe gel protected groups(0.1g/L,1g/L and 10g/L).The areas of the extravasation injuries were measured to observe the degree of doxorubicin-in...