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阿司匹林抑制肺癌细胞增殖的实验研究
引用本文:刘国华,陈燕明,冯一中. 阿司匹林抑制肺癌细胞增殖的实验研究[J]. 肿瘤防治研究, 2005, 32(11): 695-598
作者姓名:刘国华  陈燕明  冯一中
作者单位:1. 215001,南京医科大学附属苏州医院呼吸科
2. 苏州大学医学院病理学教研室
摘    要:
 目的 观察阿司匹林在体外对肺腺癌细胞A549增殖的抑制作用。方法 采用噻唑蓝(MTT)法观察阿司匹林及与奥沙利铂联用抑制A549细胞的增殖;采用流式细胞仪(FCM)观察阿司匹林处理后A549细胞周期分布的变化;采用HE染色和DNA末端原位标记染色技术(TUNEL)观察阿司匹林处理后诱导A549细胞凋亡的作用。结果 MTT显示阿司匹林呈剂量依赖方式抑制A549细胞增殖。阿司匹林作用后,FCM显示G0/G1期细胞比例增加,S期和G/M期细胞比例降低,TUNEL显示细胞凋亡指数(AI)由3.67%±1.15%增加到26.33%±2.52%,呈一定剂量效应关系。光镜下可见典型的细胞凋亡形态学变化。阿司匹林(2.5mmol/L)与奥沙利铂(≥6.25ug/mL)联用可增强抑制A549增殖的作用,二者呈协同或相加作用。结论 阿司匹林可抑制肺腺癌细胞A549的增殖,其影响细胞周期分布、诱导细胞凋亡可能是其重要的机制。阿司匹林及与奥沙利铂联用有显著的协同抗增殖效应。

关 键 词:阿司匹林  肺癌  细胞增殖  奥沙利铂
文章编号:1000-8578(2005)10-0695-04
收稿时间:2004-12-07
修稿时间:2004-12-072005-06-06

Experimental Study on Inhibitive Effecs of Aspirin on the Proliferation of Human Lung Cancer Cells
LIU Guo-hua,CHEN Yan-ming,FENG Yi-zhong. Experimental Study on Inhibitive Effecs of Aspirin on the Proliferation of Human Lung Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2005, 32(11): 695-598
Authors:LIU Guo-hua  CHEN Yan-ming  FENG Yi-zhong
Affiliation:1. Department of Resparation , The Suzhou Hospital Affiliated to Nanjing Medical University ,Suzhou 215001 , China;2. Department of Pathology , The Medical College , Suzhou University
Abstract:
Objective  To observe the inhibitive effect of aspirin on the proliferation of lung adenocarcinoma cell line A549 in vitro. Methods  MTT assay was used to observe the antiproliferative effects of aspirin ,combined with oxaliplation on A549 cells ; Flow cytometry was used to analyze the effects of aspirin on the dist ribution of A549 cells cycle , HE and the TdT-mediated d-UTP nick end-labeling assay (TUNEL) technique were used to observe the effect s of aspirin on the apoptosis of A549 cells. Results  Aspirin inhibited the proliferation of A549 cells in a dose-dependent manner. After treatment , the cell percent of G0/G1 phase increased ,and that of S and G2/ M phase decreased , A I increased from 3. 67 % ±1. 15 % to 26. 33 % ±2. 52 % with the raise of aspirin concent ration. A549 cells exhibited typical morphologic features of apoptosis by light microscopy. When aspirin (2. 5mmol/ L) and oxaliplation ( ≥6. 25 ug/mL) were combined , the inhibitory effect was enhanced in a symergistic or additive pattern. Conclusion  Aspirin can inhibit the proliferation of lung adenocarcinoma cells A549 through influencing the dist ribution of cell cycle and inducing apoptosis of A549 cells. Aspirin combined with oxaliplation shows significantly synergistic anti2tumor effect s on A549 cells.
Keywords:Aspirin  Lung cancer   Cell proliferation   Oxaliplation
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