异体垂体移植诱发ICR小鼠子宫腺肌病动物模型的建立

目的探索用ICR小鼠建立子宫腺肌病实验动物模型的可行性和有效性。方法24只7周龄已达到性成熟但未曾受孕的雌性ICR小鼠开腹行异体脑垂体子宫内移植手术,小鼠分别在垂体移植术4个月后(20只)、6个月后(4只)处死,取出子宫和卵巢称重,计算小鼠子宫湿重/终末体重的比值,同时计数子宫表面突出的子宫腺肌病结节,联合组织切片HE染色检查评估子宫腺肌病的严重程度,并与未行垂体移植雌性ICR小鼠(4只)比较。结果垂体移植术后4个月,95%的小鼠(19/20)可诱发形成子宫腺肌病。术后6个月100%小鼠(4/4)诱发形成子宫腺肌病。对照组4只小鼠即使在术后6个月也不能自发发生子宫腺肌病。术后6个月小鼠终末体重、子宫湿重以及子宫湿重/终末体重的比值垂体移植组分别为37.33±2.21g、93.00±5.66mg和2.49±0.12;未做垂体移植组分别为36.25±2.48g、79.25±9.58mg和2.18±0.13。虽然两组小鼠终末体重和子宫湿重差异均无统计学意义(P>0.05),但垂体移植组的子宫湿重/终末体重比值显著高于未行垂体移植组(t=-3.698;P=0.034)。垂体移植术后6个月,平均子宫腺肌病突起结节数为11.50±2.38个;HE染色的平均分数为3.25±0.32。结论选择ICR小鼠经垂体移植手术建立子宫腺肌病实验动物模型是行之有效的。

The development of uterine adenomyosis induced by pituitary isografting in female ICR mice

Objective:To investigate the feasibility and effectiveness of experimentally-induced uterine adenomyosis in ICR mice.Methods:A pituitary was allografted in the lumen of the right uterus of 24 virgin female ICR mice at the age of 7 weeks respectively.Four and six months after pituitary grafting,20 and 4 mice were then killed respectively and the uteri and ovaries were weighed.The right uteri were examined under a 20-fold microscope and counted for the extrauterine nodules,which was the characteristic of advanced adenomyosis,and were also examined under a 100-fold microscope to evaluate the severity of adenomyosis as compared to the controls (4 mice).Results:Four and six months after pituitary grafting,95% (19/20) and 100% (4/4) of the mouse uteri developed adenomyosis respectively.However,no evidence of adenomyosis was found in the controls.Six months after grafting,the body weight,the uterine wet weight and the ratio of uterine wet weight/body weight were 37.33±2.21g,93.00±5.66 mg and 2.49±0.12 in the pituitary grafted group,respectively,and 36.25±2.48 g,79.25±9.58 mg and 2.18±0.13 in the control group,respectively.The ratio of uterine wet weight/body weight was significantly higher in the pituitary grafted group than that in the control group (t=-3.698;P=0.034),although there was no statistically difference of the body weight and the uterine wet weight between two groups (P>0.05).Moreover,the number of extrauterine nodules detected was 11.50±2.38 and the severity of adenomyosis classification under microscopic examination was graded as 3.25±0.32 in mice six months after pituitary allografting.Conclusion:The application of ICR mice for the animal model of uterine adenomyosis developed by pituitary allografting is feasible and effective.