| 蒽酮-硫酸法测定灵芝破壁孢子粉中多糖含量的研究 |
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| 引用本文： | 许玲,李晔,黄晋之,宋玉娥. 蒽酮-硫酸法测定灵芝破壁孢子粉中多糖含量的研究[J]. 海峡药学, 2009, 21(7): 69-71 |
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| 作者姓名： | 许玲 李晔 黄晋之 宋玉娥 |
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| 作者单位： | 福建仙芝楼生物科技有限公司,福州,350002;福建仙芝楼生物科技有限公司,福州,350002;福建仙芝楼生物科技有限公司,福州,350002;福建仙芝楼生物科技有限公司,福州,350002 |
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| 摘 要： | 目的 建立灵芝破壁抱子粉中多糖含量的测定方法。方法 采用紫外分光光度法,检测波长为625nm,用葡萄糖为对照品,测定灵芝破壁抱子粉中多糖含量。结果 不同的提取方法对于多糖的提取率不同。最佳提取方法为沸水浴提取8h。结论 本法稳定可靠,简便快捷,适用于灵芝破壁抱子粉多糖含量测定。
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| 关 键 词： | 灵芝 破壁孢子粉 蒽酮-硫政法 多糖 |
Determination of polysaccharide content in sporoderm-broken Ganoderma lucidum spores by Anthrone-Sulfuric acid method |
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| XU Ling,LI Ye,HUANG Jin-zhi,SONG Yue. Determination of polysaccharide content in sporoderm-broken Ganoderma lucidum spores by Anthrone-Sulfuric acid method[J]. Strait Pharmaceutical Journal, 2009, 21(7): 69-71 |
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| Authors: | XU Ling LI Ye HUANG Jin-zhi SONG Yue |
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| Affiliation: | (Fujian Xianzhilou Biological Science and Technolog Co., Ltd. Fuzhou 350002, China) |
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| Abstract: | OBJECTIVE To develop a method for the determination of polysaccharide content in sporodermbroken Ganoderma lucidum spores. METHODS The contents of polysaccharide in sporoderm-broken Ganoderma lucidurn spores were determined by UV spectorphotometry at 625nm, with glucose being used as the reference substance. RESULTS Extraction rates of polysaccharide due to the extraction methods. Heating in a 100℃ water bath for 8 hours was found to be the optimal extraction method. CONCLUSION This method is dependable, convenient and rapid, can be used as the determination of polysaccharide in broken cellular wall spore of Ganoderma lucidum. |
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| Keywords: | Ganoderma lucidum Sporoderm-broken Spore Anthrone-sulfuric acid method Polysaccharide |
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