Properties of deproteinized bone for reparation of big segmental defect in long bone

OBJECTIVE: To explore suitable scaffold material for big segmental long bone defect by studying the properties of the prepared deproteinized bone. METHODS: Cancellated bone were made as 30 mm x mm x 3 mm bone blocks from inferior extremity of pig femur along bone trabecula. The deproteinized bone was prepared with an improved method. Their morphological features, components, cell compatibility, mechanical and immunological properties were investigated respectively. RESULTS: Deproteinized bone maintained natural reticular pore system. The main organic material is collagen I and inorganic composition is hydroxyapatite. It has good mechanical properties, cell adhesion rate and histocompatibility. CONCLUSION: This deproteinized bone can be applicable as scaffold for reparation of big segmental defect in long bone.