调节青少年特发性脊柱侧凸患者椎旁肌改变的关键微RNA

目的探寻调节青少年特发性脊柱侧凸(AIS)患者椎旁肌改变的关键微RNA(miRNA)。方法对3例女性AIS患者凹侧与凸侧椎旁肌组织进行RNA高通量测序,获得差异表达miRNA。使用27例AIS手术患者的椎旁肌组织采用实时荧光定量PCR法验证测序的质量和差异,并将miRNA的相对差异表达量与患者临床参数进行相关性分析。结果 AIS患者凹侧与凸侧椎旁肌组织存在18个差异表达miRNA。实时荧光定量PCR法检测结果显示,miR-516、miR-517、mi R-495的表达差异具有统计学意义(P 0.05)。两侧椎旁肌miR-516相对表达量差值与患者发病年龄呈负相关(r=-0.452),miR-517相对表达量差值与侧凸Cobb角呈负相关(r=-0.378)。结论 AIS患者两侧椎旁肌miR-516、miR-517、miR-495存在不对称表达,其中miR-516、miR-517的不对称表达与发病年龄和侧凸角度相关。

Key microRNA for regulation of paraspinal muscles in patients with adolescent idiopathic scoliosis

Objective To identify key microRANs(miRNAs) for adolescent idiopathic scoliosis(AIS)-associated paravertebral muscle changes.Methods RNA high-throughput sequencing was performed on 3 pairs of paravertebral muscle tissues from 3 AIS patients to obtain differentially expressed miRNAs. Paravertebral muscle tissues of 27 AIS patients were used to verify the quality and difference of miRNAs by real-time fluorescence quantitative PCR. The correlation between the relative differential expression of miRNAs and clinical parameters was analyzed.Results There were 18 differentially expressed miRNAs were found between concave and convex paravertebral muscles in the 3 AIS patients. The results of realtime fluorescence quantitative PCR showed that there were significant differences in the expression of miR-516, miR-517 and miR-495 between 2 sides of the paravertebral muscles(P < 0.05). Correlation test suggested that the difference of miR-516 expression between bilateral paraspinal muscles was negatively correlated with the age of onset(r=-0.452), while the difference of miR-516 expression was negatively correlated with the Cobb''s angle(r=-0.378).Conclusion MiR-516, miR-517 and miR-495 would be asymmetric expression between 2 sides of paravertebral muscle in AIS patients, and the asymmetric expression of miR-516 and miR-517 might be correlated with age and Cobb''s angle.