丙型肝炎病毒分片段抗体与HCVRNA的检测分析

目的探讨丙型肝炎病毒(HCV)分片段抗体(HCV-C,HCV-NS3,HCV-NS4,HCV-NS5)对丙肝的诊断价值。方法对ELISA法抗-HCV总抗体阳性的55份阳性血清及80份可疑血清再进行分片段抗体ELISA检测及RT-PCR。结果55份ELISA法抗-HCV总抗体阳性血清经分片段抗体ELISA检测后,抗-NS3、抗-C的检出率最高,分别为96.4%、89.1%;两个以上片段检测结果阳性的血清有54份,与RT-PCR结果(HCVRNA阳性52份)高度符合。80份可疑血清中10份抗-C和抗-NS3同时阳性和9份HCVRNA阳性。结论HCV分片段抗体ELISA法有很高的敏感性和特异性,与RT-PCR法基本一致。抗-NS3、抗-C在HCV的诊断中有重要的意义,抗-NS5和抗-NS4有诊断的互补作用。

The detection and analysis of different region anti-bodies and HCVRNA of HCV

Objective To discuss the diagnostic value of HCV different region anti-bodies for the diagnosis of HCV.Methods The different region anti-bodies and HCV-RNA were detected in 55 ELISA anti-HCV( ) cases and 80 questionable cases.Results The positive rates of anti-NS3 and anti-C of the 55 samples were the highest,respectively 96.4% and 89.1%.There are 54 samples which has two or more positive regions,which was in accord with the HCV RNA assays(there are 52 positive samples of HCV RNA).There are 10 samples with anti-C and anti-NS3 positive and 9 samples with HCV RNA positive.Conclusion Anti-HCV different Region ELISA has the same sensitivity and specificity as that of RT-PCR.Anti-NS3 and anti-C were very important to diagnose HCV,anti-NS5 and anti-NS4 had mutually complemented effect in diagnosis.