实验性脑出血大鼠急性期脑组织含水量与ET、NO含量的关系

目的观察大鼠脑出血后血肿周围脑组织含水量与内皮素（ET）、一氧化氮（NO）含量之间的关系,探讨ET、NO在脑出血后脑水肿形成中的作用,为临床治疗提供新的理论依据和方法。方法选用健康雄性SD大鼠48只,体重250 g~300 g,随机分为脑出血组和假手术对照组,每组24只,两组动物再随机分为术后1 d、3 d、5 d、7 d四个时间点,每个时间点6只。通过脑内注射胶原酶建立大鼠脑出血模型。测定不同时间点脑组织含水量与ET、NO含量。结果脑出血组各时间点脑组织含水量及ET、NO含量均高于对照组（P〈0.05或P〈0.01）。脑组织含水量与ET、NO含量之间呈正相关关系（P〈0.05或P〈0.01）。结论脑出血后ET、NO过量生成,且与脑水肿程度呈正相关关系。脑出血后脑水肿的发生与ET和NO过量生成有关。

Relationship between Water Content and Endothelin and Nitric Oxide in Brain Tissue at Acute Stage of Intracerebral Hemorrhage in Rats

Objective To observe the change of water content and endothelin and nitric oxide（NO）in brain tissue at acute stage of intracerebral hemorrhage（ICH）in experimental rats,and to investigate the mechanism of brain edema formation after ICH.Methods Forty-eight healthy male Sprague-Dawley rats were randomly divided into sham-operated group and ICH group.Then each group were divided into four subgroups（n=6 in each subgroup） based on different time points after operation（1 d,3 d,5 d and 7 d）.ICH rat model was established by collagenase injection to caudate nucleus.The water contents,endothelin and NO of brain tissue were detected.Results The water contents,endothelin and NO of brain tissue in ICH group were obviously higher than that of control group（P0.05 or P0.01）.The water content was positively correlated with endothelin and NO of brain tissue（P0.05 or P0.01）.Conclusion The expression of endothelin and NO in brain tissue were elevated in ICH rats,which had correlation with the level of cerebral edema.Overexpression of endothelin and NO in brain tissue might mediate the brain edema in ICH.