偏侧猴帕金森病模型的脑深部电刺激研究

目的 探讨丘脑底核脑深部电刺激(STN DBS)对纹状体多巴胺代谢的影响.方法 2只偏侧帕金森病(PD)模型猴,在电极植入术前及电刺激后1、3个月分别行单光子放射计算机断层扫描(SPECT)测定脑内多巴胺转运体(DAT)及多巴胺D2受体(D2R)的含量;利用高效液相色谱分析(HPLE-ECD)检测脑脊液中多巴胺及其代谢产物的含量和变化.结果 偏侧PD模型猴在深部脑刺激下症状明显缓解,SPECT显示在有效刺激后纹状体区DAT特异性摄取率增高,D2R特异性摄取率下降.脑脊液中多巴胺及其代谢产物的含量与术前相比无明显差异.结论 STN DBS可有效改善偏侧PD模型猴的症状.SPECT提示有效电刺激STN提高了纹状体区多巴胺代谢活性.

Deep brain stimulation of subthalamic nucleus in the hemiparkinsonian monkeys

Objective To investigate the change of striatal dopaminergic system of hemiparkinsonian monkeys treated by deep brain stimulation (DBS) in subthalamic nucleus (STN).Methods Two hemiparkinsonian monkeys were implanted simulating electrodes in right STN according to stereotaxic atlas of the monkey brain. Single photon emission computed tomography (SPECT) examination was performed at 1, 3 months after being stimulated. The dopamine and its metabolites in cerebrospinal fluid were tested by high performance liquid chromatography and electrochemical detection (HPLE-ECD). Results Symptoms of hemiparkinsonian monkeys significantly improved after STN DBS. SPECT examination indicated dopamine transporter (DAT) binding of corpus striatum in stimulated side increased, while D 2 receptor (D 2 R) decreased to the level of normal side. HPLC-ECD demonstrated that there was no change of the dopamine and its metabolites in cerebrospinal fluid compared to pre-stimulation.Conclusion After stimulation, the symptoms of hemiparkinsonian monkeys improve obviously by STN DBS. SPECT examination indicates that activity of striatal dopaminergic system may be promoted by chronic high frequency stimulation.