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| STUDY OF MUTAGENICITY OF VIRAL VECTOR IN TUMOR GENETHERAPY | |
| 引用本文： | 李贺书,李殿俊,刘旭,李大林. STUDY OF MUTAGENICITY OF VIRAL VECTOR IN TUMOR GENETHERAPY[J]. 中国癌症研究, 2002, 14(1): 24-27. DOI: 10.1007/s11670-002-0005-8 |
| 作者姓名： | 李贺书 李殿俊 刘旭 李大林 |
| 作者单位： | LI He-shu LI Dian-jun LIU Xu LI Da-lin 1Cancer Research Institute，2The Third Affiliated Hospital，Harbin Medical University，Harbin 150040 |
| 基金项目： | Foundation item: This work was supported by a grant from the Natural Science Foundation of Heilongjiang Province (D0122). |
| 摘 要： | With recent progress in tumor genetherapy, it抯 safety has been become a focus. The mutagenicity of DNA and supernatant of transgenic cells with retrovirus and adenovirus vectors was studied in this paper, which would supply clinic safety data for transgenic cells as tumor vaccines.[1] MATERIALS AND METHODS Cells Culture Recombination and packaging of human IL-2 cDNA with retrovirus vector pLXSN were completed by Shanghai Tumor Institute. Recombinant adenovirus (rAd-LacZ… |
| 收稿时间： | 2001-12-20 |
| 修稿时间： | 2002-02-02 |
Study of mutagenicity of viral vector in tumor genetherapy |
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| He-shu Li,Dian-jun Li,Xu Liu,Da-lin Li. Study of mutagenicity of viral vector in tumor genetherapy[J]. Chinese Journal of Cancer Research, 2002, 14(1): 24-27. DOI: 10.1007/s11670-002-0005-8 | |
| Authors: | He-shu Li Dian-jun Li Xu Liu Da-lin Li |
| Affiliation: | (1) Cancer Research Institute, Harbin Medical University, 150040 Harbin;(2) The Third Affiliated Hospital, Harbin Medical University, 150040 Harbin |
| Abstract: | Objective: To observe the mutagenicity of retrovirus and adenovirus as transgenic vectors to evaluate the safety of transgenic tumor cells as tumor vaccines. Methods: Cells were cultured together with the virus. Then DNA and supernatant were tested for mutagenicity by means of genetic toxicological laboratory technique. Results: The results indicated that DNA and supernatant of transgenic cells had no mutagenicity through both in vivo and in vitro tests. Conclusion: The modified virus had no mutagenicity as a transgenic vector. |
| Keywords: | Retrovirus adenovirus Tumor genetherapy Ames micronucleus |
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