反义hTR对舌鳞癌细胞系Tca8113作用的初步探讨

目的：观察反义hTR对人舌鳞癌细胞系Tca8113的影响及其与细胞周期和凋亡的关系。方法：脂质体介导真核表达载体PBBS212－hTR转染Tca8113细胞系，运用流式细胞仪技术观察细胞周期的改变和凋亡，并结合免疫组化和原位杂交技术分析转染前后某些基因表达的变化，结果：转染反义hTR的Tca8113细胞克隆生长减缓，群体倍增时间较未转染和转染空质粒组明显延长，流式细胞仪显示转染细胞PI指数下降，有亚二倍体凋亡峰出现，转染后细胞p21基因表达水平显著增高，裸鼠移植瘤实验显示转染后的Tca8113致瘤性减弱，结论：反义hTR可引起细胞通过细胞周期关卡受阻而显著抑制Tca8113细胞的生长，同时激活了某些凋亡程序而导致细胞凋亡，端粒酶可能是肿瘤基因治疗的理想靶点。

Effect of antisense hTR transfection on Tca8113 cell line

Objective To observe the apoptosis and cell cycle chang of antisense hTR transfection on Tca8113 cell line.Methods Via the mediation of lipofectAMINE2000,eukaryotic expression vecter PBBS212-hTR expressing antisense hTR expressing antisense hTR under the control of the MPSV promoter was transfected into Tca8113 cells.Cell cycle and DNA analysis was performed with FCM.Alternation of p21?hTR activity was evaluated by immunochemical method and in situ hybridization.Results Antisense hTR transfected Tca8113 reduced growth rate and increased population doubling time.Cell cycle analysis showed that the cell number of S phase and PI reduced after transfection,and an apoptotic peak was found.Anti-hTR therapy accompanied with up regulation of p21,which might take part in the cell cycle checkpoint inhibition.Conclusion Antisense hTR therapy may lead to apoptosis and cell cycle inhibition in Tca8113 cell line,it might be a promising target of tumor gene therapy.