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| 淫羊藿素对 SD 大鼠骨髓基质细胞增殖与成骨分化的影响 | |
| 引用本文： | 罗光明,顾飞飞,张迎娣,张劲娥,郭鹏女,李学智,黄远亮. 淫羊藿素对 SD 大鼠骨髓基质细胞增殖与成骨分化的影响[J]. 实用口腔医学杂志, 2016, 0(4): 467-470. DOI: 10.3969/j.issn.1001-3733.2016.04.004 |
| 作者姓名： | 罗光明 顾飞飞 张迎娣 张劲娥 郭鹏女 李学智 黄远亮 |
| 作者单位： | 1. 同济大学口腔医学院上海牙组织修复与再建工程技术研究中心, 上海,200072;2. 同济大学附属东方医院口腔科;3. 青海省西宁市第一人民医院口腔科 |
| 基金项目： | 上海市科委西医引导类项目(编号:14411963500);上海浦东新区科技发展基金创新资金(编号:PKJ2013-Y12) |
| 摘 要： | 目的：研究淫羊藿素（ICT）对体外培养 SD 大鼠骨髓基质细胞（rBMSCs）增殖与成骨分化的影响。方法：体外分离培养 rBMSCs，传代至第4代作多向分化鉴定。分别以10－9、10－8、10－7、10－6、10－5 mol／L ICT 刺激 rBMSCs 后3、6、9 d，分别用 cck-8及碱性磷酸酶 ALP 试剂盒检测 rBMSCs 的增殖及 ALP 活性；10－9 mol／L ICT 处理 rBMSCs 后21 d 作茜素红（AR）染色以判断钙结节的形成。结果：原代培养的 rBMSCs 贴壁生长、呈梭形，能多向分化；ICT 明显抑制了 rBMSCs 的增殖；但增高其 ALP 活性、钙结节形成。结论：ICT 以剂量依赖方式抑制 rBMSCs 的增殖但促进其分化和矿化。 |
| 关 键 词： | 淫羊藿素(ICT) 骨髓基质细胞(BMSCs) 增殖 分化 |
The effects of icaritin on the proliferation and osteogenic differentiation of rat bone marrow stromal cells |
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| LUO Guangming;GU Feifei;ZHANG Yingdi;ZHANG Jin’e;GUO Pengnv;LI Xuezhi;HUANG Yuanliang. The effects of icaritin on the proliferation and osteogenic differentiation of rat bone marrow stromal cells[J]. Journal of Practical Stomatology, 2016, 0(4): 467-470. DOI: 10.3969/j.issn.1001-3733.2016.04.004 | |
| Authors: | LUO Guangming GU Feifei ZHANG Yingdi ZHANG Jin’e GUO Pengnv LI Xuezhi HUANG Yuanliang |
| Affiliation: | LUO Guangming;GU Feifei;ZHANG Yingdi;ZHANG Jin’e;GUO Pengnv;LI Xuezhi;HUANG Yuanliang;School of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration;Department of Stomatology,Shanghai East Hospital Affiliated with Tongji University;Department of Stomatology,Xining First People’s Hospital of Qinghai Province; |
| Abstract: | Objective:To investigate the effects of icaritin(ICT)on the proliferation and osteogenic differentiation of rat bone mar-row stromal cells(rBMSCs).Methods:rBMSCs were cultured from the bone marrow of SD rats and identified by multilineage differ-entiation assays.3,6 and 9 days after the treatment of rBMSCs of passage 4 by ICT at 1 0 -9 ,1 0 -8 ,1 0 -7 ,1 0 -6 and 1 0 -5 mol/L re-spectively,the proliferation and differentiation of the cells were examined by cck-8 and alkaline phosphatase (ALP)activity assay kit respectively.The calcium nodule formation was observed by alizarin red(AR)staining 21 days after 1 0 -9 mol/L ICT treatment. Results:Primary rBMSCs showed the typical spindle-like shape with attachment growth.rBMSCs could be induced to osteogenic and adipogenic differentiation.The proliferation of rBMSCs was inhibited but ALP activity was enhanced by ICT.1 0 -9 mol/L ICT in-cresed calcium nodule formation.Conclusion:ICT can dose-dependently inhibit the proliferation,but promote the osteogenic differ-entiation of rBMSCs. |
| Keywords: | Icaritin(ICT) Bone marrow stromal cell(BMSCs) Proliferation Differentiation |
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