马尾松花粉硫酸酯化多糖对大鼠主动脉平滑肌细胞 [Ca2+]i调控及增殖的影响

目的 研究马尾松花粉多糖PPM60-A及其硫酸酯化物SPPM60-A对大鼠动脉平滑肌细胞 Ca²⁺]_i调控及增殖的影响。方法 常规水提醇沉法制备马尾松花粉多糖，Sephacryl S-400HR色谱分离得PPM60-A，氯磺酸-吡啶法得硫酸酯化物SPPM60-A，酯化度为1.28。酶解法分离制备大鼠动脉平滑肌细胞，测定酯化前后多糖对其胞内 Ca²⁺]_i和细胞增殖的影响。结果 PPM60-A和SPPM60-A均可以降低 Ca²⁺]_i，抑制高K⁺和去甲肾上腺素（NE）诱导的钙离子升高，降低高K⁺引起的钙离子水平上升，对NE诱导的血管主动脉平滑肌细胞增殖具有显著的抑制作用。PPM60-A作用效果好于SPPM60-A。结论 PPM60-A及SPPM60-A均能抑制细胞外Ca²⁺内流，抑制血管平滑肌细胞增殖。

Effect of sulfated polysaccharide from masson pine pollen on [Ca2+]i regulation and proliferation of arterial smooth muscle cells from rats

Objective To explore the effects of polysaccharide PPM60-A from masson pine (Pinus massoniana) pollen and its sulfated derivative SPPM60-A on Ca²⁺]_i regulation and proliferation of arterial smooth muscle cells from rats. Methods Polysaccharide (PPM60-A) was extracted by hot water, classified by 60% ethanol from masson pine pollen and purified by Sephacryl S-400HR. Sulfated polysaccharide (SPPM60-A) was obtained from PPM60-A modified by chlorosulfonic acid-pyridin method and its esterification degree is 1.28. The original generation of vascular smooth muscle cells (VSMCs) was obtained by enzyme digestion with collagenae from artery of rats. The effects of PPM60-A and SPPM-60A on Ca²⁺]i regulation and cell proliferation were determined. Results Both PPM60-A and SPPM60-A could decrease the Ca²⁺]_i significantly, inhibit the rising of calcium ion concentration induced by KCl and noradrenalin (NE); reduce the calcium ion level pretreated by KCl; inhibit the VSMCs proliferation induced by NE. Conclusion PPM60-A and SPPM60-A could inhibit the influx of calcium ion and the proliferation of VSMCs.