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| Livin siRNA重组腺病毒的构建及其对K562细胞增殖、凋亡和耐药的影响 | |
| 引用本文： | 郭玥馨,毛成刚,罗庆,郑改焕,徐酉华. Livin siRNA重组腺病毒的构建及其对K562细胞增殖、凋亡和耐药的影响[J]. 第三军医大学学报, 2009, 31(14) |
| 作者姓名： | 郭玥馨 毛成刚 罗庆 郑改焕 徐酉华 |
| 作者单位： | 1. 重庆医科大学附属儿童医院血液肿瘤科,重庆,400014 2. 重庆医科大学附属儿童医院儿科研究所外科实验室,重庆,400014 |
| 摘 要： | 目的 通过改良的重组腺病毒介导方法 研究Livin基因与K562细胞增殖、凋亡及耐药的关系.方法 用基因工程技术将靶向Livin siRNA片段克隆至穿梭质粒pSES-HUS中,然后与骨架质粒pAdeasy同源重组,将重组成功的质粒用脂质体介导的方法 转染HEK293细胞,乒乓感染收获高滴度的重组腺病毒AdS-Livin siRNA,并将其感染K562细胞,采用Real-time PCR、Western blot检测Livin基因的干扰效果,用MTT和流式细胞仪检测其与K562细胞增殖、凋亡及多药耐药的关系.结果 成功构建重组腺病毒Ad5-Livin siRNA,并感染K562细胞,在mRNA和蛋白2个水平均抑制Livin的表达.证实通过重组腺病毒沉默Livin基因,可抑制K562细胞增殖,促进凋亡,增加其对阿霉素、长春新碱和依托泊苷的敏感性.结论 用重组腺病毒介导的方法 抑制K562细胞中Livin基因的表达,可增加K562细胞的凋亡及对抗癌药物的敏感性.Livin可能成为治疗白血病新的分子靶点. |
| 关 键 词： | 重组腺病毒 RNA干扰 Livin基因 K562细胞 耐药 |
Construction of recombinant adenovirus vector of Livin siRNA and its effects on proliferation,apoptosis and drug resistance of K562 cells |
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| GUO Yue-xin,MAO Cheng-gang,LUO Qing,ZHENG Gai-huan,XU You-hua. Construction of recombinant adenovirus vector of Livin siRNA and its effects on proliferation,apoptosis and drug resistance of K562 cells[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(14) | |
| Authors: | GUO Yue-xin MAO Cheng-gang LUO Qing ZHENG Gai-huan XU You-hua |
| Affiliation: | GUO Yue-xin1,MAO Cheng-gang1,LUO Qing2,ZHENG Gai-huan1,XU You-hua1(1Department of Hematology and Tumor,2Laboratory of Surgery,Institute of Pediatrics Children's Hospital,Chongqing Medical University,Chongqing 400014,China) |
| Abstract: | Objective To study the effect of recombinant adenovirus vector-mediated Livin siRNA on the proliferation,apoptosis and drug resistance in K562 cells.Methods The targeting Livin siRNA was subcloned into the shuttle plasmid pSES-HUS,and then recombinant it with adenovirus skeleton plasmid pAdeasy by homologous recombination.We transfected the product into HEK293 cells via liposome and generated recombinant adenovirus Ad5-Livin siRNA.K562 cells were infected with Ad5-Livin siRNA.The gene silencing efficiency o... |
| Keywords: | recombinant adenovirus RNA interference Livin K562 cells drug resistance |
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