CD147反义RNA抑制神经胶质瘤侵袭的实验研究

目的 探讨CD147在人脑胶质瘤侵袭中的作用机制. 方法构建 CD147反义RNA表达质粒并转染人胶质瘤细胞系U251(转染组),同时设空载体对照组和空白对照组,经筛选、鉴定后应用明胶酶谱实验和重组基底膜实验分别检测3组细胞中基质金属蛋白酶(MMPs)的分泌和侵袭细胞的数量. 结果与空载体转染组和空白对照组比较,CD147反义RNA转染组U251细胞产生MMPs的能力下降,侵袭细胞的数量降低,差异有统计学意义(P<0.05). 结论 CD147反义核酸能抑制胶质瘤的侵袭浸润,提示其可能成为胶质瘤治疗的药物靶点.

Role of CD147 in glioblastoma progression and the inhibitory effect of its antiseuse RNA on tumor invasion and angiogenesis

Objective To investigate the role of CD147 in glioma invasion, metastasis, and angiogenesis. Methods Glioma celt fine U251 was transfectzd with a CD147 antisense RNA expression vector. After selection and identification of the transfected cells, gelatin zymography, scrape-wound migration assay and Matrigel Boyden chamber assay were performed to analyze the inhibitory effects on the invasiveness and angiogenesis of the glioblastoma cells. Enzyme-linked immunosorbent assay (ELISA) was used to detect vascular endothelial growth factor (VEGF) level in the supernatant of the transfected cells. Result Gelatin zymography showed that MMP-9 and MMP-2 production was decreased after antisense RNA transfection of the cells in comparison with the blank control and empty vector-transfected U251 cells. Matrigel Boyden chamber assay demonstrated a significant reduction in the invasiveness of the transfected cells. Conclusion CD147 antisense RNA can inhibit the invasion, metastasis, and angiogenesis of glioma cells in vitro, suggesting the potential of CD147 as a new target for tumor therapy.