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| 耐甲氧西林金黄色葡萄球菌PFGE分型研究 | |
| 引用本文： | 王磊,祁伟,孔秀凤,宋诗铎. 耐甲氧西林金黄色葡萄球菌PFGE分型研究[J]. 天津医药, 2011, 39(8): 714-716. DOI: 10.3969/j.issn.0253-9896.2011.08.015 |
| 作者姓名： | 王磊 祁伟 孔秀凤 宋诗铎 |
| 作者单位： | 1. 天津医科大学第二医院感染风湿科2. 天津医科大学第二医院感染性疾病研究所3. 天津医大二院感染研究所4. 天津医科大学第二医院 天津市感染性疾病研究所 |
| 摘 要： | 目的:检测不同医院耐甲氧西林金黄色葡萄球菌(MRSA)优势菌株是否相同。方法:用常规方法对从天津医科大学第二医院及武汉同济医院临床标本中分离的金黄色葡萄球菌进行鉴定,采用头孢西丁纸片扩散法及mecA基因检测筛选出MRSA。采用脉冲场凝胶电泳法(PFGE)进行分型,以了解不同地区两家医院的优势菌株是否相同。结果:共分离出金黄色葡萄球菌127株,MRSA83株,其中天津56株,武汉27株。天津56株MRSA临床株PFGE分型为15型27个亚型,以A1型为主;武汉27株MRSA临床株PFGE分型为6型12个亚型,以A2、A4型为主。结论:2家医院MRSA优势菌株并不相同,尚未发现MRSA流行菌株。 |
| 关 键 词： | 耐甲氧西林金黄色葡萄球菌 |
| 收稿时间： | 2010-09-06 |
| 修稿时间： | 2010-12-08 |
Methicillin Resistant Staphylococcus Aureus Typing with Pulsed-Field Gel Electrophoresis |
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| WANG Lei,QI Wei,KONG Xiufeng,SONG Shiduo. Methicillin Resistant Staphylococcus Aureus Typing with Pulsed-Field Gel Electrophoresis[J]. Tianjin Medical Journal, 2011, 39(8): 714-716. DOI: 10.3969/j.issn.0253-9896.2011.08.015 | |
| Authors: | WANG Lei QI Wei KONG Xiufeng SONG Shiduo |
| Affiliation: | WANG Lei,QI Wei,KONG Xiufeng,SONG Shiduo Institute of Infectious Diseases & Infection and Rheumatism department,The Second Hospital of Tianjin Medical University,Tianjin 300211,China |
| Abstract: | Objective:To study whether there was the same dominance strain of methicillin resistant staphylococcus aureus(MRSA) obtained from two hospitals.Methods:According to routine process,clinical strains were identified from Tongji Hospital in Wuhan and the Second Hospital of Tianjin Medical University.MRSA was screened through cefoxitin disc diffusion test and the detection of mecA gene.The clinical strains were typed with pulsed-field gel electrophoresis,and the prevalence strain was checked.Results:There were ... |
| Keywords: | methicillin resistant staphylococcus aureus electrophoresis gel pulsed-field cefoxitin microbial sensitivity tests |
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