机械拉伸波形及频率对肺腺癌细胞增殖的影响

通过FX-4000柔性基底加载系统研究了不同波形及频率的周期性机械拉伸对人肺腺癌A 549细胞株增殖的影响,应用Im age-P ro图像处理软件对A 549细胞株在心形波、三角波及方波等拉伸应变下的增殖动力学变化进行了分析。实验表明:在co llagenⅠ基底膜上,应变0-25%,频率为20次/m in、40次/m in及60次/m in,加载时间为2 h时,与对照组比较,方波刺激组细胞生长明显受到抑制,三角波刺激组细胞增殖率无明显差异,心形波刺激组细胞增殖加快。研究表明:A 549细胞株对体外的生理应变作出响应时,方波与60次/m in频率的组合刺激抑制作用最佳,且加载时间越长抑制效果越好。可见,波形与频率的合理组合在抑制人肺腺癌细胞增殖的过程中起着重要作用。

Mechanical Strain of Different Waveform and Frequency Inhibits Proliferation of Lung Adenocarcinoma Cell in Vitro

The effects of waveform and frequency on proliferation of human lung adenocarcinoma cell line A549 were studied by FX-4000 strain unit . The results showed that cellular proliferation index (PI) reduced significantly in squarewave group when compared with the control after A549 cells were subjected to collagen I 0-25% elongation at frequency 20 cycles/min, 40 cycles/min and 60 cycles/min for 2h. The PI showed no distinct difference in trianglewave group. The PI increased in heartwave group. These data indicate that A549 cells do respond to physiological strain in vitro. The effects of squarewave and 60 cycles/min tension may be optimal for inhibiting the proliferation of A549 cells. To select appropriate waveform and frequency will be of paramount importance to inhibiting proliferation of A549 cells.