| HPLC法测定养阴降糖胶囊中哈巴苷和哈巴俄苷的含量 |
| |
| 引用本文： | 陈登丰,何孝金.HPLC法测定养阴降糖胶囊中哈巴苷和哈巴俄苷的含量[J].中国医院用药评价与分析,2014(8):703-705. |
| |
| 作者姓名： | 陈登丰 何孝金 |
| |
| 作者单位： | 福建中医药大学附属南平市人民医院;南平市药品检验所 |
| |
| 摘 要： | 目的：建立同时测定养阴降糖胶囊中哈巴苷和哈巴俄苷含量的方法。方法：色谱柱采用C18柱（4.6 mm×100 mm,5μm）;流动相为乙腈-0.03%磷酸,梯度洗脱;检测波长为210 nm;流速为1.0 ml/min。结果：哈巴苷、哈巴俄苷进样量分别在19.240~384.800 ng（r=1.000 0）、17.568~351.360 ng（r=1.000 0）范围内与峰面积线性关系良好,平均回收率分别为（98.33±1.85）%（RSD=1.88%）、（97.43±1.13）%（RSD=1.37%）。结论：该方法简单、准确、专属性强,可用于养阴降糖胶囊的质量控制。
|
| 关 键 词： | 养阴降糖胶囊 哈巴苷 哈巴俄苷 含量测定 |
Content Determination of Harpagide and Harpagoside in Yangyin Hypoglycemic Capsule by HPLC |
| |
| CHEN Deng-feng;HE Xiao-jin.Content Determination of Harpagide and Harpagoside in Yangyin Hypoglycemic Capsule by HPLC[J].Evaluation and Analysis of Drug-Use in Hospital of China,2014(8):703-705. |
| |
| Authors: | CHEN Deng-feng;HE Xiao-jin |
| |
| Institution: | CHEN Deng-feng;HE Xiao-jin;Fujian College of Traditional Chinese Medicine Affiliated Nanping Municipal People’s Hospital;Nanping Institute of Drug Control; |
| |
| Abstract: | OBJECTIVE：To establish a method for simultaneous determination of harpagide and harpagoside in Yangyin hypoglycemic capsules. METHODS：The determination was performed using C18column（4.6 mm×100 mm,5 μm）with mobile phase consisting of acetonitrile-0.03% phosphoric acid by gradient elution program;the detection wavelength was set at 210 nm and the flow rate was set at 1.0 ml/min. RESULTS：The linear ranges of harpagide and harpagoside were 19.240-384.800 ng（r=1.000 0）and 17.568-351.360 ng（r=1.000 0）,respectively. The average recovery rates were（98.33±1.85）%（RSD=1.88%）and（97.43±1.13）%（RSD=1.37%）,respectively. CONCLUSIONS：The method is simple,feasible,accurate,reliable and specific and it can be used for quality control of Yangyin hypoglycemic capsule. |
| |
| Keywords: | Yangyin hypoglycemic capsules Harpagide Harpagoside Content determination |
| 本文献已被 CNKI 维普 等数据库收录！ |
