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| 青石棉诱发大鼠胸膜间皮瘤 | |
| 引用本文： | 杨青,罗素琼,刘学泽,詹承烈. 青石棉诱发大鼠胸膜间皮瘤[J]. 现代预防医学, 1995, 0(4) |
| 作者姓名： | 杨青 罗素琼 刘学泽 詹承烈 |
| 作者单位： | 华西医科大学公共卫生学院 |
| 基金项目： | 国家教委高校博士点科研基金,CMB资助 |
| 摘 要： | 本文采用右胸腔内注入染尘法，对大鼠染青石棉尘４０ｍｇ。收集１９例胸膜间皮瘤标本，提取标本中ＤＮＡ，用人工合成的寡核苷酸引物行ＰＣＲ体外扩增，再用ｒ－（３２）ＰＡＴＰ标记正常和两种空变Ｋｉ－ｒａｓ１２位探针，杂交，放射自显影。结果表明，７例正常对照组标本Ｋｉ－ｒａｓ１２位点突变为阴性；１９例间皮瘤标本中，２例突变阳性，其中１例由ＧＧＴ（Ｇｌｙ）突变为ＡＧＴ（Ｓｅｒ），１例由ＧＧＴ（Ｇｌｙ）突变为ＴＧＴ（Ｃｙｓ）。 |
| 关 键 词： | 青石棉，间皮瘤，癌基因 |
STUDIE OF Ki-ras MUTATION TESTS IN RAT MESOTHELIOMA INDUCED BY CROCIDOLITE. |
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| Yang Qing,et al.. STUDIE OF Ki-ras MUTATION TESTS IN RAT MESOTHELIOMA INDUCED BY CROCIDOLITE.[J]. Modern Preventive Medicine, 1995, 0(4) | |
| Authors: | Yang Qing et al. |
| Abstract: | mesothelioma samples were taken.The normal and two kinds of mutation Ki-ras l2 codon probe were end-labeled with r-(32)PATP.The DNA amplified in vitro by PCR was spotted onto the Nylon filters.The filters were hybridized and exposed to x-ray films.The results showed that 2 among 19 mesothelioma specimens in Ki-ras 12 codon mutation tests were positive.Of these one changed from GGT(Gly)to AGT(Ser),the other changed from GGT (Gly)to TGT(Cys). |
| Keywords: | Crocidolite Mesothelioma Oncogene |
| 本文献已被 CNKI 等数据库收录！ | |