两种纳米形貌对MG63成骨细胞增殖、分化的影响

目的:评价钛表面纳米管和纳米颗粒形貌对MG63成骨细胞的增殖、分化能力的影响。方法:通过磁控溅射和阳极氧化技术制备纳米颗粒和纳米管形貌。采用扫描电镜和轮廓仪表征材料表面形貌以及粗糙度,并将MG63成骨细胞株与不同形貌的钛材料进行复合培养,检测1d、4d、7d的MTT值及4d、7d的碱性磷酸酶活性。结果:培养1d、4d、7d后,纳米管和纳米颗粒组MTT值高于对照组,7d时,纳米管组MTT值高于纳米颗粒组;培养7d后,纳米形貌实验组碱性磷酸酶活性明显高于对照组。结论:纳米管和纳米颗粒形貌均有利于成骨细胞的增殖和分化。相对于纳米颗粒,表面的纳米管形貌更有利于促进成骨细胞的增殖。

Influence of Two Nanotopography on MG63 Osteoblast Proliferation and Differentiation

Objective: To investigate the influence of nanotopologies on MG63 osteoblast proliferation and differentiation.Methods: The nanoparticle topography was deposited by magnetron sputtering and nanotube was prepared by anodizing method.The topography was observed by scanning electron microscope(SEM) and the surface roughness was calculated by surface profiler.MG64 cells were cultured on samples with different topographies.The cell proliferation was investigated with MTT assay after 1 d,4 d and 7 d.The activity of alkaline phosphatase(ALP) was measured after 4 d and 7 d.Results: MTT test showed that the proliferation of osteoblasts on the two nanotopography surfaces was significantly improved than that on flat surface after 1d,4d and 7d.The proliferation level of the nanotube group was significantly higher than that of nanoparticle group after 7d.The ALP activity level on nanotopography surfaces was significantly higher than that on flat surface.Conclusion: The nanotube and nanoparticle topography had a positive effect on osteoblast proliferation and differentiation.The nanotube may be more conducive to improve cell proliferation than nanoparticle topography.