新型骨支架复合材料修复牙槽骨缺损研究

目的:探讨多孔聚丙烯晴基碳纤维/壳聚糖/双相磷酸钙/聚乳酸-羟基乙酸(PAN/CS/BCP/PLGA)复合支架对大白兔牙槽骨缺损的修复能力。方法:采用真空冷冻干燥技术制备多孔PAN/CS/BCP/PLGA复合支架。选取32只成年雄性新西兰大白兔,随机分为实验组和对照组,手术制备大小为10mm×10mm×10mm牙槽骨缺损区。实验组骨缺损区植入PAN/CS/BCP/PLGA复合支架,对照组植入CS/PLGA复合材料,术后2、4、8、12周分期取材,进行X线检查、组织学观察、扫描电镜(SEM)检测和成骨细胞计数分析。结果:术后12周实验组检测结果,X线观察:骨缺损区阻射影与自体骨相同。组织学观察:骨缺损区完全被新骨充填,且为较成熟的板层骨,与自体骨完全融合。SEM检测:骨-材料界面形成骨性结合,移植材料大部分降解,被成熟骨组织替代。成骨细胞计数分析:成骨细胞生长活跃,统计学有显著性差异。结论:多孔PAN/CS/BCP/PLGA复合支架具备良好的生物活性和生物可降解性,对骨缺损的修复能力要优于CS/PLGA复合材料,为其成为骨组织工程支架替代材料提供理论依据。

Research on New Bone Stents Composite Materials to Repair Alveolar Bone Defect

Objective: To investigate the repair ability of porous polyacrylonitrile-based carbon fiber/chitosan/biphasic calcium phosphate/poly lactic acid-glycolic acid(PAN/CS/BCP/PLGA) composite scaffold transplanted onto the alveolar bone defect of rabbit.Methods: The porous PAN/CS/BCP/PLGA composite scaffold was prepared by vacuum freeze-drying technique.32 adult male New Zealand big white rabbit were divided into the experimental group and the control group.Alveolar bone defeat area of 10mm×10mm×10mm was prepared.The experimental group was transplanted with PAN/CS/BCP/PLGA composite scaffold and the control group was transplanted with CS/PLGA,followed by observation in 2,4,8,and 12 weeks,respectively,for radiologic analysis,scanning electron microscope(SEM) and osteoblast count analysis.Results: 12 weeks after the test results,radiographic evaluation showed the resistance felt at the bone defect area is similar to auto-grafting bone.Histologic observation showed that the bone defect area is completely filled with new bone,with is mature shelf bone,and fully merged with auto-grafting bone.The SEM results showed bone formation at bone-material interface,which is replaced with mature bone tissue.Analysis of osteoblast count showed active growth of osteoblast with statistically significant difference.Conclusion: The porous PAN/CS/BCP/PLGA composite scaffold has good biological activity and biodegradable nature,and is better than CS/PLGA composite materials for repairing bone defects,providing a theoretical basis for using alternative materials as bone tissue engineering scaffolds.