| MAG-1对人肺癌细胞转移能力的影响及其在24例肺癌组织中的表达 |
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| 引用本文： | 张金强,孟宇宏,蔺会云,刘刚,王妍,陈惠华,徐元基,杜芝燕,陆应麟.MAG-1对人肺癌细胞转移能力的影响及其在24例肺癌组织中的表达[J].中国肺癌杂志,2009,12(2). |
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| 作者姓名： | 张金强 孟宇宏 蔺会云 刘刚 王妍 陈惠华 徐元基 杜芝燕 陆应麟 |
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| 作者单位： | 1. 军事医学科学院基础医学研究所病理生物学研究室,北京,100850
2. 海军总医院病理科,北京,100037 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金面上项目 |
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| 摘 要： | 背景与目的 肺癌转移相关基因-1(Pulmonary cancer Metastasis Associated Gene-1,MAG-1)是利用抑制消减杂交技术获得的与肺癌转移相关的基因.此研究的目的在于探讨基因MAC-1对肺巨细胞癌细胞的转移相关生物学行为的影响及其在24例肺癌组织中的表达情况.方法 构建MAG-1的正反义表达载体,分别转染PLA801C和PLA801D,获得稳定表达相应序列的细胞株,观察稳定转染细胞的多种肿瘤转移相关能力的改变,并检测MAG-1对CD44、MMP-2等分子的影响,最后还检测TMAG-1在源于不同转移状况患者的肺癌标本中的表达情况.结果 MAG-1对细胞生长、细胞与胞外基质的粘附能力以及侵袭能力均有促进作用;并能促进细胞中CD44和MMP-2的表达;MAC-1 mRNA在有转移的肺癌中的阳性率(7/12)高于其在无转移的肺癌标本中的阳性率(2/12).结论 MAG-1具有增强肺癌细胞转移能力的功能,并且在转移性肺癌组织中其mRNA具有较高的阳性检出率,是可能的肺癌转移相关基因.
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| 关 键 词： | 肿瘤转移 肺肿瘤 基质金属蛋白酶-2 |
Promotion of MAG-1 on Metastasis of Lung Cancer Cells in vitro and Its Expression in Lung Cancer Tissue of 24 Cases |
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| Jinqiang ZHANG,Yuhong MENG,Huiyun LIN,Gang LIU,Yan WANG,Huihua CHEN,Yuanji XU,Zhiyan DU,Yinglin LU.Promotion of MAG-1 on Metastasis of Lung Cancer Cells in vitro and Its Expression in Lung Cancer Tissue of 24 Cases[J].Chinese Journal of Lung Cancer,2009,12(2). |
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| Authors: | Jinqiang ZHANG Yuhong MENG Huiyun LIN Gang LIU Yan WANG Huihua CHEN Yuanji XU Zhiyan DU Yinglin LU |
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| Institution: | Jinqiang ZHANG*,Yuhong MENG*#,Huiyun LIN*,Gang LIU*,Yan WANG*,Huihua CHEN*,Yuanji XU*,Zhiyan DU*,Yinglin LU* *Department of Pathobiology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China,#Present address:Department of Pathology,Navy General Hospital,Beijing 100037 |
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| Abstract: | Background and objective Tumor metastasis is a multistep process with many genes involved in.A novel gene MAG-1,identified by suppression subtractive hybridization from lung cancer cells was found to be associated with tumor metastasis.The aims of this work are to investigate the metastasis related effects of MAG-1 on human lung-giant-cell line PLA801,and to compare the expression rate of MAG-1 in cancer tissue from lung cancer patients with different metastatic status.Methods Sense and anti-sense expressin... |
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| Keywords: | CD44 |
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