荧光薄层扫描法测定卫胃颗粒中盐酸小檗碱的含量

目的:建立卫胃颗粒中盐酸小檗碱的含量测定方法。方法:样品经提取、薄层展开后,采用荧光扫描法对卫胃颗粒中盐酸小檗碱进行含量测定,展开剂为苯-乙酸乙酯-甲醇-异丙醇-水(6:3:1.5:1.5:0.3),激发波长λ=366nm。结果:盐酸小檗碱在0.03104～0.1552μg范围内呈良好的线性关系,回收率为100.8％,RSD=1.9％。结论:测定方法简单、灵敏,结果准确、重现性好,可用于本品的质量控制。

Determination of Berberine Hydrochioride in Weiwei Granules by TLC-Fluorometry

Objective: To establish a determining method for a content of berberine hydrochloride in Weiwei granules. Method: The sample was extracted and developed, the berberine hydrochloride was determinated by TLC-Fluoromety with benzene-ethyl ace-late-methanol-isobutanol-water( 6:3-1.5:1.5:0.3)asa developing solvent system and X = 366nm as scanning wavelength. Result: The berberine hydrochloride had a good linear relationship between 0.03104 and 0.1552 /ig.the average recovery was 100.8% ,RSD = 1.9% .Conclusion:The method is simple, sensitive, and the result is accurate with a good reproducibility, it can be used for quality control of this preparation.