小檗胺通过内质网应激通路诱导胃癌细胞A GS凋亡

目的:研究小檗胺对人胃癌细胞AGS增殖和凋亡的影响,并探讨其可能的作用机制.方法:应用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法测定细胞活力;膜联蛋白V/碘化丙啶(An-nexin V/PI)细胞凋亡法测定细胞凋亡;Western blot法测定Bcl-2、Bax、chop、GRP78和cle...

Berbamine induces the apoptosis of gastric cancer AGS cells via endoplasmic reticulum stress pathway

Objective:To investigate the effects and the possible mechanisms of berbamine on the cell proliferation and apoptosis of human gastric cancer AGS cells.Methods:Cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Cell apoptosis was detected by Annexin V/PI staining assay.The expression of Bcl-2,Bax,chop,GRP78 and cleaved caspase 12 proteins was detected by Western blot assay.Results:MTT assay result showed that berbamine could obviously inhibit the cell growth of AGS cells in a dosage-dependent manner.The cell apoptosis assay result showed that berbamine significantly increased the apoptotic rate of AGS cells.The Western blot result showed that berbamine down-regulated the expression of Bcl-2,while up-regulated the expression of Bax,chop,GRP78 and cleaved caspase 12 proteins.Moreover,compared with the berbamine group,the expression of chop in berbamine+chop siRNA group significantly decreased(P<0.01),while the expression of Bcl-2 in berbamine+chop siRNA group significantly increased(P<0.01).Conclusion:Berbamine could induce cell apoptosis via endoplasmic reticulum stress pathway in gastric cancer AGS cells.