ALMS 1-IT1在结直肠癌中的表达及其对癌细胞增殖、侵袭和上皮间质转化的影响

目的:探讨lncRNA ALMS1-IT1在结直肠癌(colorectal cancer,CRC)中的表达及预后意义,明确ALMS1-IT1对CRC细胞增殖、侵袭和上皮间质转化(epithelial-mesenchymal transition,EMT)的影响.方法:通过GEPIA网络平台分析癌症基因图谱(The Ca...

The expression of ALMS1-IT1 in colorectal cancer and its effect on cancer cell proliferation,invasion and epithelial-mesenchymal transition

Objective:To explore the expression and prognostic significance of lncRNA ALMS1-IT1 in colorectal cancer(CRC)and determine the impact of ALMS1-IT1 in CRC cell proliferation,invasion and epithelial-mesenchymal transition(EMT).Methods:The expression of ALMS1-IT1 in CRC and its correlation with the survival of patients were analyzed by GEPIA web server using The Cancer Genome Atlas(TCGA)database.The expression of ALMS1-IT was detected by RT-qPCR in human normal intestinal epithelial cells(NCM460)and CRC cells(HCT116,HT29,LoVo,SW480).ALMS1-IT was silenced and overexpressed by plasmid transfection in LoVo and SW480 cells,respectively.CCK-8 assay detected cell proliferation ability.Transwell assay analyzed cell migration and invasion potentials.Immunoblotting was used to detect the changes of EMT markers(E-cadherin,N-cadherin,Vimentin).Results:TCGA data analysis confirmed that ALMS1-IT expression in CRC tissues was significantly higher than that in normal tissues,and high expression of ALMS1-IT1 was prominently associated with poor prognosis.The expression of ALMS1-IT1 in CRC cells(HCT116,HT29,LoVo,SW480)was markedly higher than that in normal intestinal epithelial cells(NCM460).ALMS1-IT1 silencing inhibited cell proliferation,migration,invasion,and led to increased expression of E-cadherin protein and decreased expression of N-cadherin and Vimentin protein in LoVo cells.Overexpression of ALMS1-IT1 promoted cell proliferation,migration,invasion,and resulted in decreased expression of E-cadherin protein and increased expression of N-cadherin and Vimentin protein in SW480 cells.Conclusion:ALMS1-IT1 is highly expressed in CRC tissues and is closely related to poor prognosis.ALMS1-IT1 exerts an oncogenic role by promoting the proliferation,invasion and EMT of CRC cells.