五倍子水提取物去除根管内玷污层的扫描电镜观察

目的:观察五倍子对根管内玷污层的去除效果。方法:72个离体单根牙随机分为9组,冲洗液为五倍子组(分别为200、100、50、25、12.5、6.25mg/mL)、30mL/L过氧化氢液加生理盐水组、170g/LEDTA加52.5g/LNaOCl组(阳性对照)、双蒸水组(阴性对照)。根管预备后用各冲洗液冲洗,选根中部分进行扫描电镜观察。样本分为10个区,分别评分,结果进行统计学分析。结果:170g/LEDTA加52.5g/LNaOCl组去除玷污层效果最好,但管周牙本质有过度脱矿现象。25、50、100、200mg/mL五倍子组去除玷污层的效果较好,其去除玷污层的能力随浓度增加而增大,且牙本质小管有缩小的现象,当大于50mg/mL时牙本质小管被堵塞。30mL/L过氧化氢液加生理盐水组和双蒸水组不能去除玷污层。结论:大于6.25mg/mL的五倍子水提取物有去除玷污层的作用,随浓度增大,牙本质小管缩小并出现堵塞。

A scannig electron microscopic study on the effect of chinese nutgall for smear layer removal on root canal walls

AIM:To observe the effect of Chinese nutgall for smear layer removal on root canal. METHODS: Seventy-two human teeth with one root canal were randomly divided into nine groups. The irrigates were Chinese nutgall(200mg/mL, 100mg/mL, 50mg/mL, 25mg/mL, 12.5mg/mL and 6.25mg/mL), 3 mL/L hydrogen peroxide plus physiological saline, 170g/L EDTA plus 52.5g/L NaClO (positive control group) and distilled water (negative control group). Each group was prepared and irrigated, and the middle of canal walls was observed with scanning electron microscopy. A scoring system for smear layer was used to analysis. RESULTS: The most completed smear layer removal was showed in the group of 170g/L EDTA plus 52.5g/L NaClO, but erosion of the dental tubule occurred. Chinese nutgall with 25mg/ml, 50mg/ml, 100mg/ml and 200mg/ml effectively removed the smear layer, and the degree of the smear layer removal increased with the rise of the concentration. An contraction action on the dental tubules was found. The dental tubules were blocked when concentration was more than 50mg/mL. The group of 30mL/L hydrogen peroxide plus physiological saline and distilled water could not remove the smear layer. CONCLUSION: Chinese nutgall with concentration above 6.25mg/ml can remove the smear layer effectively. It also seemed to have an contraction and block action on dental tubules with concentration increased.