体外联合应用IL-10和甲基强的松龙处理树突状细胞诱导移植的免疫耐受

目的探讨体外联合应用白细胞介素10(IL-10)和甲基强的松龙(Medron)处理供体树突状细胞(DC)对小鼠皮肤移植术后免疫耐受的诱导效果,为抗移植术后免疫排斥反应治疗提供依据。方法以健康成年C57BL/6小鼠为供体。BALB/c小鼠为受体,随机分为11组。除A组外,其余各组均于皮肤移植前3d自尾静脉输入对应的供体DC。具体对应关系如下A组为空白对照,尾静脉输入生理盐水;B组为输入未修饰的DC;C1组为10μg/LIL-10处理组;C2组为30μg/LIL-10处理组;D1组为10mg/LMedron处理组;D2组为20mg/LMedron处理组;E1~E4组分别为10μg/LIL-10 10mg/LMedron处理组、10μg/LIL-10 20mg/LMedron处理组、30μg/LIL-10 10mg/LMedron处理组、30μg/LIL-10 20mg/LMedron处理组。同时设立F组,为BALB/c对BALB/c的同种同基因皮片移植。各组行皮肤移植术,观察受体移植皮片存活情况。结果相对于生理盐水组,E3组(30μg/LIL-10 10mg/LMedron处理组)的移植皮片存活时间最长,经统计学分析两种药物之间具有交互作用(P<0.05)。结论用IL-10和修饰的供体树突状细胞对受体进行预处理,可明显延长移植皮片的存活时间。

Immune tolerance induced by IL-10 and methylprednisolone modified dendritic cells in vitro

AIM: To study the effect on induction of allograft immune tolerance by IL-10 and methylprednisolone (Medron) modified dendritic cells(DC) of donor in murine allogenetic skin transplantation model. METHODS: The recipient BALB/c mice were divided into 11 groups.Except group A, each group had been injected with corresponding donor DC through vena caudalis, and transplanted full-thickness skin through back to back from donor C57BL/6 three days later. Corresponding doses were: A: injected NS, blank control; B: injected non-modified donor DC; C1: 10 microg/L IL-10 group; C2: 30 microg/L IL-10 group; D1: 10 mg/L Medron group; D2: 20 mg/L Medron group; E1-E4: 10 microg/L IL-10+10 mg/L Medron group, 10 microg/L IL-10+20 mg/L Medron group, 30 microg/L IL-10+10 mg/L Medron group and 30 microg/L IL-10+20 mg/L Medron group. F group was skin transplantation between two BALB/c mice. Skin transplantation was performed and the survival time of skin allografts was observed. RESULTS: Among all the groups, the graft of E3 group(30 microg/L IL-10 mg/L Medron) survived for the longest time. Statistical analysis showed that there was interaction between IL-10 and Medron (P<0.05). CONCLUSION: The pretreatment with donor DC treated by IL-10 and Medron can prolong the survival time of murine skin allografts after transplantation and induce immune tolerance successfully.