中药刺五加中总皂苷的微波辅助提取方法研究

目的:建立中药刺五加中总皂苷微波辅助提取方法。方法:用微波辅助提取法提取中药刺五加中总皂苷。利用正交实验,考察了微波提取条件(包括溶剂、微波辐射时间、提取压力和料液比)对刺五加中总皂苷提取率的影响规律,优化了最佳提取条件。结果:在所选因素水平下,溶剂为70%乙醇、提取压力为700kPa、提取时间为10min、料液比为1:30时,刺五加中总皂苷提取效率最佳。与传统的索氏提取法进行了比较研究,微波辅助提取效率可提高34%。结论:微波辅助提取法提取中药刺五加中总皂苷,提取时间短,提取效率高。

Microwave - assisted extraction of total saponins from Acanthopanax senticosus Harms

Objective:To develop a high performance microwave-assisted extraction (MAE)method of saponins compounds from Acanthopanax senticosus Harms.Methods:The total saponins in Acanthopanax senticosus Harms have been extracted by means of MAE technique.The influence of some operation conditions,such as ethanol con- tent,microwave irradiation time,processing pressure and solvent uptake rate,on the extraction yield have been stud- ied by using orthogonal test and optimization based on multivariate statistics.Results:The highest extraction yield could be obtained when extraction solvent was 70% ethanol,processing pressure was 700 kPa,microwave irradiation time was 10 minutes and the ratio of sample weight to solvent volume was 1:30.In the optimal conditions of MAE, the extraction yield of total saponins was higher 34% than that with Soxhlet extraction.Conclusion:MAE is more advantageous in respect of extraction time and extraction yield.