注射用灯盏花素大鼠和豚鼠免疫毒性差异性比较

目的 研究注射用灯盏花素在两种不同的动物种属及其反应体系上的免疫毒性差异.方法 按照《中药、天然药物免疫毒性（过敏性、光变态反应）研究的技术指导原则》的试验方法对注射用灯盏花素进行SD大鼠皮肤被动过敏试验和Hartley豚鼠全身主动过敏试验,比较注射用灯盏花素在大鼠和豚鼠体内的免疫毒性差异.结果 注射用灯盏花素剂量为5 mg/kg、50 mg/kg及500 mg/kg时均未引起SD大鼠皮肤被动过敏反应阳性;注射用灯盏花素剂量为5 mg/kg（豚鼠等效剂量）时引起50％动物弱阳性和50％动物阳性,50 mg/kg时豚鼠全身主动过敏反应阳性率为100％.结论 注射用灯盏花素在两种动物体内表现的过敏反应存在着明显的差异,豚鼠全身主动过敏反应相对于SD大鼠皮肤被动过敏反应更敏感,与临床过敏反应发生率更具有一致性.

Comparison on Immunotoxicity Difference of Breviscapine Injection between Rat and Guinea Pig

Objective To study the immunotoxicity difference of Breviscapine Injection between rat and guinea pig. Methods According to the Chinese traditional medicine, natural medicine immunity toxicity study technical directed principle, the PCA and ASA tests were carded out in order to compare the immunotoxicity difference between the two animals. Results The results of the allergy testing showed that no positive symptom in SD rat occurred at all the doses of 5 mg/kg, 50 mg/kg and 500 mg/kg of Breviscapine injection group in PCA toxicity study. While in ASA toxicity study, 50 percent animals appeared weak positive and 50 percent animals appeared positive at the dose of 5 mg/kg of Breviscapine injection group; and all appeared the positive symptoms at the high dose of 50 mg/kg group. Conclusions Compared with the allergic response in animal study, there are significant difference between the PCA and the ASA toxicity study and the ASA is more sensitive and more conformity to the application in clinical.