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| 生理状态下外周血白细胞γ-干扰素基因表达的荧光定量PCR检测 | |
| 引用本文： | 王茹,陈佩杰,董强刚. 生理状态下外周血白细胞γ-干扰素基因表达的荧光定量PCR检测[J]. 中国运动医学杂志, 2005, 24(6): 693-695 |
| 作者姓名： | 王茹 陈佩杰 董强刚 |
| 作者单位： | 上海体育学院运动科学系,上海,200438 |
| 基金项目： | 科技部专项基金，上海市重点学科建设项目，上海市重点学科建设项目，教育部优秀青年教师资助计划，上海市体育局资助项目 |
| 摘 要： | 目的:建立γ-干扰素(IFN-γ)基因表达的绝对定量实时PCR检测技术。方法:应用实时定量PCR方法检测25名健康运动员生理状态下外周血白细胞IFN-γmRNA的表达。主要结果和结论:应用实时定量PCR方法能够对健康受试者外周血白细胞自发性IFN-γmRNA表达进行定量分析,相关系数r>0.99。 |
| 关 键 词： | γ-干扰素 信使核糖核酸 实时定量PCR |
| 修稿时间： | 2004-12-10 |
Quantitative Real-time Detection of Spontaneous Interferon-γ Gene Expression in Peripheral Blood Mononuclear Cell (PBMC) by Polymerase Chain Reaction |
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| Wang Ru,Chen Peijie,Dong Qianggang. Quantitative Real-time Detection of Spontaneous Interferon-γ Gene Expression in Peripheral Blood Mononuclear Cell (PBMC) by Polymerase Chain Reaction[J]. Chinese Journal of Sports Medicine, 2005, 24(6): 693-695 | |
| Authors: | Wang Ru Chen Peijie Dong Qianggang |
| Abstract: | Objective To develop a method for quantitative real-time detection of interferon-γ(IFN-γ) using polymerase chain reaction (PCR). Methods Gene expression of IFN-γ in peripheral blood mononuclear cell (PBMC) from 25 healthy individuals was detected by PCR. An endogenous “housekeeping” gene GAPDH was used to normalize the results. Results and Conclusion The spontaneous IFN-γ mRNA expression in PBMC in a given sample could be measured by PCR with relativity correlation coefficient of r>0.99. |
| Keywords: | IFN-γ mRNA real-time PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |