Comparison of different root canal sealers: cytotoxicity and the type of induced cell death |
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| Authors: | Valentina Brzovic Ivana Miletic Ivica Anic Lidija Vukovic Lumnije Kqiku Maja Osmak |
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| Affiliation: | (1) Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Croatia;(2) Division of Molecular Biology, Laboratory for Genotoxic Agents, Rudjer Boskovic Institute, Zagreb, Croatia;(3) Division of Preventive and Operative Dentistry, Endodontics, Pedodontics, and Minimally Invasive Dentistry, Medical University of Graz, Austria |
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| Abstract: | Summary The aim of this study was to compare in vitro cytotoxicity and determine the type of cell death induced by Hermetic, Diaket, GuttaFlow, Epiphany, SuperEBA and IRM using
human laryngeal carcinoma HEp2 cells as the model system. The cytotoxicity was determined by counting the total number of
cells by electronic counter, while the number of viable cells was estimated under a light microscope. The number of apoptotic
or necrotic cells was estimated under a fluorescent microscope according to their characteristic morphology and staining with
specific fluorescent dyes. The results show that GuttaFlow was biocompatible, hardly reducing the number of living human laryngeal
carcinoma HEp2 cells through all research periods (except after one month period). On the contrary, Diaket, Epiphany, IRM,
Hermetic, and Super EBA exhibited strong toxicity. Diaket and IRM mostly induced apoptosis while for Epiphany, Hermetic and
Super EBA, necrosis was the predominant or only type of cell death.
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| Keywords: | Apoptosis Cytotoxicity Necrosis Root canal sealers |
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