Role of interleukin-1 and prostaglandin in in vitro bone resorption induced by Actinobacillus actinomycetemcomitans lipopolysaccharide |
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| Authors: | Yuichi Ishihara Tatsuji Nishihara Eijiro Maki Toshihide Noguchi Toshihiko Koga |
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| Affiliation: | Department of Dental Research, National Institute of Health, Shinagawa-ku, Tokyo, Japan;Department of Periodontology, School of Dentistry, Aichigakuin University, Chikusa-ku, Nagoya, Japan |
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| Abstract: | ![]()
Lipopolysaccharide (Y4 LPS) isolated from Actinobacillus actinomycetemcomitans strain Y4 induced bone resorption in BALB/c mouse calvaria organ culture. The calcium release from LPS-low responsive C3H/HeJ mouse calvaria by Y4 LPS was very low. Indomethacin almost completely inhibited prostaglandin E2 (PGE2) production by Y4 LPS-stimulated BALB/c mouse calvaria, but did not suppress interleukin-1 (IL-1) release from the calvaria, and partially suppressed the bone resorption. Dexamethasone strongly inhibited the PGE2 and IL-1 production by Y4 LPS-stimulated BALB/c mouse calvaria. as well as Y4 LPS-induced bone resorption. Dexamethasone inhibited expression of membrane IL-1 on osteoblastic cells stimulated with Y4 LPS, but indomethacin did not. Furthermore, anti-IL-1 serum partially suppressed the calcium release from Y4 LPS-stimulated BALB/c mouse calvaria. These results suggest that both PGE2 and IL-1 participate in Y4 LPS-induced bone resorption in vitro . |
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| Keywords: | interleukin-1 prostaglandin A. actinomycetemcomitans LPS bone resorption |
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