粉防己碱对人结肠癌细胞株HT-29增殖与凋亡的影响

目的:通过观察粉防己碱对人结肠癌细胞株HT-29增殖与凋亡的影响,初步探讨粉防己碱对结肠癌的体外抗肿瘤效应。方法:采用MTT比色法观察粉防己碱对HT-29细胞的增殖抑制效应,利用细胞DNA琼脂糖凝胶电泳及细胞凋亡荧光染色法检测粉防己碱诱导肿瘤细胞凋亡的作用,以免疫细胞化学法检测粉防己碱对Bcl-2和BAX表达水平的影响。结果:MTT比色法显示粉防己碱对人结肠癌细胞株HT-29增殖有抑制作用,其抑制效应具有剂量依赖的特点,细胞凋亡荧光染色法、琼脂糖凝胶电泳表明粉防己碱可诱导HT-29细胞凋亡,免疫细胞化学法显示粉防己碱上调BAX基因表达,下调Bcl-2基因表达。结论:粉防己碱对人结肠癌细胞株HT-29增殖的抑制效应具有剂量依赖性,并可诱导细胞凋亡,其抗肿瘤效应可能与凋亡相关基因表达的调控有关。

Effect of tetrandrine on the proliferation and apoptosis of human colon cancer cell line HT-29

Objective To investigate the antitumor action of tetrandrine in human colon cancer in vitro by observing the effects of tetrandrine on the proliferation and apoptosis of cancer cell line HT-29. Methods The inhibitory action of tetrandrine in the proliferation of HT-29 cells was observed by MTT assay. Apoptosis was examined by acridine orange (AO) / ethidiumbromide (EB) fluorescent staining and DNA gel electrophoresis. The expressions of Bcl-2 and BAX were detected by immunohistochemical staining mothod. Results Tetrandrine inhibited the HT-29 cells in a dose-dependent fashion, induced the apoptosis of the HT-29 cells, upregulated BAX expression, and downregulated Bcl-2 expression. Conclusions Tetrandrine inhibits the proliferation of HT-29 in a dose-dependent manner and induces apoptosis. The antitumor effect of tetrandrine may be associated with its regulation of the expressions of the apoptosis-related genes.